Summary of 205-Shen-CellPatterning

SSBD:database
SSBD:database URL
Title
Images and BDML file for quantiative information about mouse skeletal myoblasts C2C12 adhered on a surface with the native or cross-linked albumin structure for several days.
Description
-
Relase date
2022-03-31
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
5 ( Image datasets: 5, Quantitative data datasets: 0 )
Size of Datasets
1.2 GB ( Image datasets: 1.2 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus
Cell lines(s)
C2C12
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
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Molecular Function (MF)
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Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
confocal microscopy
X scale
-
Y scale
-
Z scale
-
T scale
1 day per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Yigang Shen, Nobuyuki Tanaka, Hironori Yamazoe, Shunsuke Furutani, Hidenori Nagai, Takayuki Kawai, Yo Tanaka (2020) Flow analysis on microcasting with degassed polydimethylsiloxane micro-channels for cell patterning with cross-linked albumin., PloS one, Volume 15, Number 5, pp. e0232518

Published in 2020 (Electronic publication in May 20, 2020, midnight )

(Abstract) Patterned cell culturing is one of the most useful techniques for understanding the interaction between geometric conditions surrounding cells and their behaviors. The authors previously proposed a simple method for cell patterning with an agarose gel microstructure fabricated by microcasting with a degassed polydimethylsiloxane (PDMS) mold. Although the vacuum pressure produced from the degassed PDMS can drive a highly viscous agarose solution, the influence of solution viscosity on the casting process is unknown. This study investigated the influences of micro-channel dimensions or solution viscosity on the flow of the solution in a micro-channel of a PDMS mold by both experiments and numerical simulation. It was found experimentally that the degassed PDMS mold was able to drive a solution with a viscosity under 575 mPa.s. A simulation model was developed which can well estimate the flow rate in various dimensions of micro-channels. Cross-linked albumin has low viscosity (1 mPa.s) in aqueous solution and can undergo a one-way dehydration process from solution to solid that produces cellular repellency after dehydration. A microstructure of cross-linked albumin was fabricated on a cell culture dish by the microcasting method. After cells were seeded and cultivated on the cell culture dish with the microstructure for 7 days, the cellular pattern of mouse skeletal myoblast cell line C2C12 was observed. The microcasting with cross-linked albumin solution enables preparation of patterned cell culture systems more quickly in comparison with the previous agarose gel casting, which requires a gelation process before the dehydration process.
(MeSH Terms)

Contact
Yo Tanaka , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Integrated Biodevice
Contributors
Yigang Shen,Nobuyuki Tanaka


Dataset List of 205-Shen-CellPatterning

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 7528
Dataset Kind Image data
Dataset Size 148.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7529
Dataset Kind Image data
Dataset Size 260.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7530
Dataset Kind Image data
Dataset Size 260.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7531
Dataset Kind Image data
Dataset Size 260.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7532
Dataset Kind Image data
Dataset Size 260.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data