Summary of 192-Hiroshima-SingleMolDyn

SSBD:database
SSBD:database URL
Title
Single-molecule images of living cells with epidermal growth factor (EGF) stimulation by automated in-cell single-molecule imaging system (AiSIS)
Description
-
Relase date
2022-03-31
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
60 ( Image datasets: 60, Quantitative data datasets: 0 )
Size of Datasets
1.5 GB ( Image datasets: 1.5 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Cricetulus griseus
Cell lines(s)
CHO-K1
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
-
Molecular Function (MF)
epidermal growth factor receptor binding
Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
fluorescence microscopy
X scale
0.072 micrometer/pixel
Y scale
0.072 micrometer/pixel
Z scale
-
T scale
33 microsecond per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Michio Hiroshima, Masato Yasui, Masahiro Ueda (2020) Large-scale single-molecule imaging aided by artificial intelligence., Microscopy (Oxford, England), Volume 69, Number 2, pp. 69-78

Published in 2020 Apr 8

(Abstract) Single-molecule imaging analysis has been applied to study the dynamics and kinetics of molecular behaviors and interactions in living cells. In spite of its high potential as a technique to investigate the molecular mechanisms of cellular phenomena, single-molecule imaging analysis has not been extended to a large scale of molecules in cells due to the low measurement throughput as well as required expertise. To overcome these problems, we have automated the imaging processes by using computer operations, robotics and artificial intelligence (AI). AI is an ideal substitute for expertise to obtain high-quality images for quantitative analysis. Our automated in-cell single-molecule imaging system, AiSIS, could analyze 1600 cells in 1 day, which corresponds to approximately 100-fold higher efficiency than manual analysis. The large-scale analysis revealed cell-to-cell heterogeneity in the molecular behavior, which had not been recognized in previous studies. An analysis of the receptor behavior and downstream signaling was accomplished within a significantly reduced time frame and revealed the detailed activation scheme of signal transduction, advancing cell biology research. Furthermore, by combining the high-throughput analysis with our previous finding that a receptor changes its behavioral dynamics depending on the presence of a ligand/agonist or inhibitor/antagonist, we show that AiSIS is applicable to comprehensive pharmacological analysis such as drug screening. This AI-aided automation has wide applications for single-molecule analysis.
(MeSH Terms)

Contact
Michio Hiroshima , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Cell Signaling Dynamics
Contributors
Michio Hiroshima


Dataset List of 192-Hiroshima-SingleMolDyn

#
Dataset ID
Kind
Size
4D View
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# 7423
Dataset Kind Image data
Dataset Size 25.0 MB
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# 7424
Dataset Kind Image data
Dataset Size 25.0 MB
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# 7425
Dataset Kind Image data
Dataset Size 25.0 MB
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# 7426
Dataset Kind Image data
Dataset Size 25.0 MB
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# 7427
Dataset Kind Image data
Dataset Size 25.0 MB
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# 7428
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
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# 7429
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
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# 7430
Dataset Kind Image data
Dataset Size 25.0 MB
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# 7431
Dataset Kind Image data
Dataset Size 25.0 MB
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# 7432
Dataset Kind Image data
Dataset Size 25.0 MB
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# 7433
Dataset Kind Image data
Dataset Size 25.0 MB
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# 7434
Dataset Kind Image data
Dataset Size 25.0 MB
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# 7435
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7436
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7437
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7438
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7439
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7440
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7441
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7442
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7443
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7444
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7445
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7446
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7447
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7448
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7449
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7450
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7451
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7452
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7453
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7454
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7455
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7456
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7457
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7458
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7459
Dataset Kind Image data
Dataset Size 25.0 MB
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SSBD:OMERO
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# 7460
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7461
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7462
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7463
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7464
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7465
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7466
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7467
Dataset Kind Image data
Dataset Size 25.0 MB
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SSBD:OMERO
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# 7468
Dataset Kind Image data
Dataset Size 25.0 MB
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SSBD:OMERO
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# 7469
Dataset Kind Image data
Dataset Size 25.0 MB
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SSBD:OMERO
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# 7470
Dataset Kind Image data
Dataset Size 25.0 MB
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SSBD:OMERO
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# 7471
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7472
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7473
Dataset Kind Image data
Dataset Size 25.0 MB
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SSBD:OMERO
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# 7474
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7475
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7476
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7477
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7478
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7479
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7480
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7481
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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# 7482
Dataset Kind Image data
Dataset Size 25.0 MB
4D view
SSBD:OMERO
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