SSBD:database

Thumbnail for 180-Watabe-Biosensoractivity

Summary of 180-Watabe-Biosensoractivity

SSBD:database
180-Watabe-Biosensoractivity
SSBD:database URL
https://ssbd.riken.jp/database/180-Watabe-Biosensoractivity/
SSBD:repository
ssbd-repos-000180
SSBD:repository URL
https://ssbd.riken.jp/repository/180/
Title
Imges of different biosensors activity expressed in HeLa cells, MDCK cells or mice ear skin.
Description
-
Release date
2022-03-31
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
115 ( Image datasets: 115, Quantitative data datasets: 0 )
Size of Datasets
3.9 GB ( Image datasets: 3.9 GB, Quantitative data datasets: 0 bytes )
Organism(s)
Homo sapiens, Canis lupus familiaris, Mus musculus
Cell lines(s)
MDCK cell, HeLa cell
Protein name(s)
ERK, JNK, PKA, ROCK, bPAC
Protein tag(s)
mCherry
Reporter(s)
AKAR3EV, Booster- PKA, Booster-ERK, Booster-JNK, Booster-PKA, Booster-ROCK2, EKAREV
Datatype
-
Molecular Function (MF)
extracellular signal-regulated kinase activity
Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
FRET, differences in fluorescence lifetime, time lapse microscopy, fluorescence microscopy
X scale
0.0176398 centimetre/pixel, 0.31 micrometer/pixel, 1.242 micrometer/pixel, 0.621 micrometer/pixel, NA, 0.994 micrometer/pixel
Y scale
0.0176398 centimetre/pixel, 0.31 micrometer/pixel, 1.242 micrometer/pixel, 0.621 micrometer/pixel, NA, 0.994 micrometer/pixel
Z scale
1 micrometer/slice, 6 micrometer/slice, NA, 3 micrometer/slice
T scale
62 sec per time interval, 124 sec per time interval, 58 sec per time interval, 60 sec per time interval, 120 sec per time interval, 125 sec per time interval, 1 sec per time interval, 59 sec per time interval, 57 sec per time interval, 1 min per time interval, 122 sec per time interval, 60.47 sec per time interval, NA, 142 sec per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Related paper(s)
Contact
Kenta Terai , Graduate School of Medicine, Kyoto University , Department of Pathology and Biology of Diseases
Contributors

Dataset List of 180-Watabe-Biosensoractivity

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#
Dataset ID
Kind
Size
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure5B_ISO_HeLa_Isotiteration_thumb_w2FRET
# 7261
Datast ID Figure5B_ISO_HeLa_Isotiteration_thumb_w2FRET
Dataset Kind Image data
Dataset Size 794.4 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure5B_ISO_HeLa_Isotiteration_thumb_w3mKO2
# 7262
Datast ID Figure5B_ISO_HeLa_Isotiteration_thumb_w3mKO2
Dataset Kind Image data
Dataset Size 794.2 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure5B_ISO_HeLa_Isotiteration_thumb_w4mKate2-FRET
# 7263
Datast ID Figure5B_ISO_HeLa_Isotiteration_thumb_w4mKate2-FRET
Dataset Kind Image data
Dataset Size 798.8 KB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure5B_ISO_HeLa_Isotiteration_thumb_w5DIC-fast
# 7264
Datast ID Figure5B_ISO_HeLa_Isotiteration_thumb_w5DIC-fast
Dataset Kind Image data
Dataset Size 798.8 KB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure5B_ISO_HeLa_Isotiteration_w1CFP
# 7265
Datast ID Figure5B_ISO_HeLa_Isotiteration_w1CFP
Dataset Kind Image data
Dataset Size 140.5 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure5B_ISO_HeLa_Isotiteration_w2FRET
# 7266
Datast ID Figure5B_ISO_HeLa_Isotiteration_w2FRET
Dataset Kind Image data
Dataset Size 140.5 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure5B_ISO_HeLa_Isotiteration_w3mKO2
# 7267
Datast ID Figure5B_ISO_HeLa_Isotiteration_w3mKO2
Dataset Kind Image data
Dataset Size 140.5 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure5B_ISO_HeLa_Isotiteration_w4mKate2-FRET
# 7268
Datast ID Figure5B_ISO_HeLa_Isotiteration_w4mKate2-FRET
Dataset Kind Image data
Dataset Size 140.5 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure5B_ISO_HeLa_Isotiteration_w5DIC-fast
# 7269
Datast ID Figure5B_ISO_HeLa_Isotiteration_w5DIC-fast
Dataset Kind Image data
Dataset Size 140.5 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6B_bPAC_44934589_thumb_w2mKO2_s1
# 7270
Datast ID Figure6B_bPAC_44934589_thumb_w2mKO2_s1
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6B_bPAC_44934589_thumb_w2mKO2_s2
# 7271
Datast ID Figure6B_bPAC_44934589_thumb_w2mKO2_s2
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6B_bPAC_44934589_thumb_w2mKO2_s3
# 7272
Datast ID Figure6B_bPAC_44934589_thumb_w2mKO2_s3
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6B_bPAC_44934589_thumb_w3mKate2-FRET_s1
# 7273
Datast ID Figure6B_bPAC_44934589_thumb_w3mKate2-FRET_s1
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6B_bPAC_44934589_thumb_w3mKate2-FRET_s2
# 7274
Datast ID Figure6B_bPAC_44934589_thumb_w3mKate2-FRET_s2
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6B_bPAC_44934589_thumb_w3mKate2-FRET_s3
# 7275
Datast ID Figure6B_bPAC_44934589_thumb_w3mKate2-FRET_s3
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6B_bPAC_44934589_w2mKO2_s1
# 7276
Datast ID Figure6B_bPAC_44934589_w2mKO2_s1
Dataset Kind Image data
Dataset Size 361.3 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6B_bPAC_44934589_w2mKO2_s2
# 7277
Datast ID Figure6B_bPAC_44934589_w2mKO2_s2
Dataset Kind Image data
Dataset Size 361.3 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6B_bPAC_44934589_w2mKO2_s3
# 7278
Datast ID Figure6B_bPAC_44934589_w2mKO2_s3
Dataset Kind Image data
Dataset Size 361.3 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6B_bPAC_44934589_w3mKate2-FRET_s1
# 7279
Datast ID Figure6B_bPAC_44934589_w3mKate2-FRET_s1
Dataset Kind Image data
Dataset Size 361.3 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6B_bPAC_44934589_w3mKate2-FRET_s2
# 7280
Datast ID Figure6B_bPAC_44934589_w3mKate2-FRET_s2
Dataset Kind Image data
Dataset Size 361.3 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6B_bPAC_44934589_w3mKate2-FRET_s3
# 7281
Datast ID Figure6B_bPAC_44934589_w3mKate2-FRET_s3
Dataset Kind Image data
Dataset Size 361.3 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6C_mKate2FRET_raw_311-326
# 7282
Datast ID Figure6C_mKate2FRET_raw_311-326
Dataset Kind Image data
Dataset Size 32.1 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure6C_mKOk_raw_311-326
# 7283
Datast ID Figure6C_mKOk_raw_311-326
Dataset Kind Image data
Dataset Size 32.1 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure7D_EarSkin_dbcAMP
# 7284
Datast ID Figure7D_EarSkin_dbcAMP
Dataset Kind Image data
Dataset Size 64.9 KB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure7D_EarSkin_dbcAMP_01
# 7285
Datast ID Figure7D_EarSkin_dbcAMP_01
Dataset Kind Image data
Dataset Size 65.2 KB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure7D_EarSkin_dbcAMP_02
# 7286
Datast ID Figure7D_EarSkin_dbcAMP_02
Dataset Kind Image data
Dataset Size 64.9 KB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure7D_EarSkin_dbcAMP_03
# 7287
Datast ID Figure7D_EarSkin_dbcAMP_03
Dataset Kind Image data
Dataset Size 64.9 KB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure7E_Crypt_dbcAMP
# 7288
Datast ID Figure7E_Crypt_dbcAMP
Dataset Kind Image data
Dataset Size 62.8 KB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure7E_Crypt_dbcAMP_01
# 7289
Datast ID Figure7E_Crypt_dbcAMP_01
Dataset Kind Image data
Dataset Size 62.9 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Figure7E_Crypt_dbcAMP_02
# 7290
Datast ID Figure7E_Crypt_dbcAMP_02
Dataset Kind Image data
Dataset Size 62.9 KB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4514NES
# 7291
Datast ID FigureS2_HeLa_4514NES
Dataset Kind Image data
Dataset Size 464.5 KB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4514NES_FSK
# 7292
Datast ID FigureS2_HeLa_4514NES_FSK
Dataset Kind Image data
Dataset Size 468.7 KB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4515NES
# 7293
Datast ID FigureS2_HeLa_4515NES
Dataset Kind Image data
Dataset Size 464.5 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4515NES_FSK
# 7294
Datast ID FigureS2_HeLa_4515NES_FSK
Dataset Kind Image data
Dataset Size 468.7 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4516NES
# 7295
Datast ID FigureS2_HeLa_4516NES
Dataset Kind Image data
Dataset Size 464.5 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4516NES_FSK
# 7296
Datast ID FigureS2_HeLa_4516NES_FSK
Dataset Kind Image data
Dataset Size 468.7 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4517NES
# 7297
Datast ID FigureS2_HeLa_4517NES
Dataset Kind Image data
Dataset Size 464.5 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4517NES_FSK
# 7298
Datast ID FigureS2_HeLa_4517NES_FSK
Dataset Kind Image data
Dataset Size 468.7 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4518NES
# 7299
Datast ID FigureS2_HeLa_4518NES
Dataset Kind Image data
Dataset Size 464.5 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4518NES_FSK
# 7300
Datast ID FigureS2_HeLa_4518NES_FSK
Dataset Kind Image data
Dataset Size 468.7 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4519NES
# 7301
Datast ID FigureS2_HeLa_4519NES
Dataset Kind Image data
Dataset Size 464.5 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4519NES_FSK
# 7302
Datast ID FigureS2_HeLa_4519NES_FSK
Dataset Kind Image data
Dataset Size 468.7 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4520NES
# 7303
Datast ID FigureS2_HeLa_4520NES
Dataset Kind Image data
Dataset Size 464.5 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4520NES_FSK
# 7304
Datast ID FigureS2_HeLa_4520NES_FSK
Dataset Kind Image data
Dataset Size 468.7 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4521NES
# 7305
Datast ID FigureS2_HeLa_4521NES
Dataset Kind Image data
Dataset Size 464.5 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4521NES_FSK
# 7306
Datast ID FigureS2_HeLa_4521NES_FSK
Dataset Kind Image data
Dataset Size 468.7 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4522NES
# 7307
Datast ID FigureS2_HeLa_4522NES
Dataset Kind Image data
Dataset Size 464.5 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4522NES_FSK
# 7308
Datast ID FigureS2_HeLa_4522NES_FSK
Dataset Kind Image data
Dataset Size 468.7 KB
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4523NES
# 7309
Datast ID FigureS2_HeLa_4523NES
Dataset Kind Image data
Dataset Size 464.5 KB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigureS2_HeLa_4523NES_FSK
# 7310
Datast ID FigureS2_HeLa_4523NES_FSK
Dataset Kind Image data
Dataset Size 468.7 KB
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