Summary of 129-Komatsu-ERKBiosensorActivity

SSBD:database
SSBD:database URL
Title
Timelapse images and BDML file for quantitative information about the extracellular signal-regulated kinase (ERK) biosensor activity in HeLa cells, or 4T1 cancer cells or the transgenic mice
Description
-
Relase date
2021-09-30
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
13 ( Image datasets: 13, Quantitative data datasets: 0 )
Size of Datasets
6.4 GB ( Image datasets: 6.4 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus
Cell lines(s)
4T1 cell, HeLa cell
Gene symbol(s)
NA
Protein name(s)
Cry2
Protein tag(s)
mCherry

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
activation of MAPERK kinase
Cellular Component (CC)
plasma membrane
Biological Imaging Method
time lapse microscopy
X scale
0.662 micrometer/pixel, Arbitrary unit by manual variable magnification., 0.867 micrometer/pixel
Y scale
0.662 micrometer/pixel, Arbitrary unit by manual variable magnification., 0.867 micrometer/pixel
Z scale
NA, 1 micrometer/slice
T scale
2 minites for each time interval, 1 minite for each time interval, 4 minites for each time interval, 121.91 second for each time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Naoki Komatsu, Kenta Terai, Ayako Imanishi, Yuji Kamioka, Kenta Sumiyama, Takashi Jin, Yasushi Okada, Takeharu Nagai, Michiyuki Matsuda (2018) A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging., Scientific reports, Volume 8, Number 1, pp. 8984

Published in 2018 Jun 12 (Electronic publication in June 12, 2018, midnight )

(Abstract) Genetically encoded biosensors based on the principle of Forster resonance energy transfer comprise two major classes: biosensors based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET). The FRET biosensors visualize signaling-molecule activity in cells or tissues with high resolution. Meanwhile, due to the low background signal, the BRET biosensors are primarily used in drug screening. Here, we report a protocol to transform intramolecular FRET biosensors to BRET-FRET hybrid biosensors called hyBRET biosensors. The hyBRET biosensors retain all properties of the prototype FRET biosensors and also work as BRET biosensors with dynamic ranges comparable to the prototype FRET biosensors. The hyBRET biosensors are compatible with optogenetics, luminescence microplate reader assays, and non-invasive whole-body imaging of xenograft and transgenic mice. This simple protocol will expand the use of FRET biosensors and enable visualization of the multiscale dynamics of cell signaling in live animals.

Contact
Michiyuki Matsuda , Kyoto University , Graduate School of Biostudies , Laboratory of Bioimaging and Cell Signaling
Contributors


Dataset List of 129-Komatsu-ERKBiosensorActivity

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 5089
Dataset Kind Image data
Dataset Size 67.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5090
Dataset Kind Image data
Dataset Size 8.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5091
Dataset Kind Image data
Dataset Size 24.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5092
Dataset Kind Image data
Dataset Size 24.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5093
Dataset Kind Image data
Dataset Size 36.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5094
Dataset Kind Image data
Dataset Size 36.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5095
Dataset Kind Image data
Dataset Size 83.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5096
Dataset Kind Image data
Dataset Size 972.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5097
Dataset Kind Image data
Dataset Size 972.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5098
Dataset Kind Image data
Dataset Size 1.2 GB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5099
Dataset Kind Image data
Dataset Size 972.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5100
Dataset Kind Image data
Dataset Size 972.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5101
Dataset Kind Image data
Dataset Size 1.2 GB
4D view
SSBD:OMERO
Download BDML
Download Image data