Summary of 123-Yalikun-ZygoteDyn

SSBD:database
SSBD:database URL
Title
Living image of capturing the whole surface of a mouse zygote during rotation
Description
-
Relase date
2019-11-20
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
2 ( Image datasets: 2, Quantitative data datasets: 0 )
Size of Datasets
542.3 MB ( Image datasets: 542.3 MB, Quantitative data datasets: 0 bytes )

Organism(s)
M. musculus
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
zygote rotation
Molecular Function (MF)
Biological Process (BP)
Fertilization
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
XY: 0.5818 micrometer/pixel, Z: 10 micrometer/slice
T scale
0.01667 second for each time interval

Image Acquisition
Experiment type
Other
Microscope type
BrightfieldMicroscope
Acquisition mode
Other
Contrast method
Darkfield
Microscope model
KCM-Z KENKO
Detector model
Lu 075C-IO, Lumenera
Objective model
-
Filter set
-

Related paper(s)

Yalikun, Yaxiaer, Aishan, Yusufu, Mosha, Abulaiti, Sumiyama, Kenta, Tanaka, Yo (2018), Oocyte all-surfaces’ imaging method using micro-scale rotational flow, Micro & Nano Letters, Volume 13, Number 3, 306-311

Published in 2018/03/01

(Abstract) In this work, the authors report an all-surfaces? image capturing method of a single oocyte that uses micro-scale rotational flow. Here, the authors used this method gently and indirectly rotated the single pronuclear zygotes (physical properties are similar to those of oocytes) to obtain images of all its surfaces in 0.33 s. The necessary equipment to realise this method consists of only a syringe pump and a microfluidic chip with a single 40 µm diameter orifice. Assessment of viability and quality of oocytes or embryos during the process of intra cytoplasmic sperm injection) and in vitro fertilisation is important for good embryo development. At present, manual morphological evaluation is the most common method to assess viability and quality of oocytes. Usually, during the manual morphological evaluation, the operator is required to manipulate the oocyte to achieve its full surface image. However, manipulation is difficult, even for an experienced operator. Conventional methods using the principles of mechanics, electronics, or magnetism require a complex system. Conventional methods using principles of fluids or optics also have limitations about target size and transparency. Here, a method is proposed and proved of using rotational flow to gently capture and rotate a mouse zygote without using a complex control system and achieved its full surface image in 0.33 s. The method offers a new tool for morphological evaluation of assessment of viability and quality of oocytes.

Contact
Yo Tanaka, Yaxiaer Yalikun , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Integrated Biodevice
Contributors
Yaxiaer Yalikun, Yusufu Aishan, Abulaiti Mosha, Kenta Sumiyama, Yo Tanaka


Dataset List of 123-Yalikun-ZygoteDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 4865
Datast ID Beads_rotation
Dataset Kind Image data
Dataset Size 329.8 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 4866
Dataset Kind Image data
Dataset Size 212.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data