Dataset 37-Arata-MolDynPar2/pmPAR-2
Author reviewedProject name
Release date
2017-10-03
Update date
2020-02-03
Pre-release URL
None
Project title
None
Contact name
Yukinobu Arata, Yasushi Sako
Organization
RIKEN
Department
Wako
Laboratory
Cellular Informatics Laboratory
License
Contributors
Yukinobu Arata, Michio Hiroshima, Chan-Gi Pack, Ravikrishna Ramanujam, Fumio Motegi, Kenichi Nakazato, Yuki Shindo, Paul W. Wiseman, Hitoshi Sawa, Tetsuya J. Kobayashi, Hugo B. Brandão, Tatsuo Shibata, Yasushi Sako
Method summary
See details in Arata et al. (2016) Cell Reports, 16, 1-13.
Paper(s)
File or Folder
pmPAR-2
Kind
Quantitative data
Title
Time-lapse semi-TIRF microscopy images of fluorescent particles of GFP::phospho-mimicPAR-2 in a C. elegans wild-type embryo
Description
-
Organism
Caenorhabditis elegans
(
NCBITaxon:6239
)
Strain
None
Datatype
single molecule dynamics
Basedon
Experiment
LocalID
pmPAR-2
XYZ-scale
XY: 0.0667 micrometer/pixel, Z: NA
T-scale
0.05 second for each time interval
dblink
None
Source
None
BDML
None
PDPML
None
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
TIRF
Contrast method
Fluorescence
Microscope model
Nikon TE2000
Detector model
Hamamatsu Photonics ImagEM
Objective model
Nikon Apo TIRF 60X/1.49
Filter set
Dichroic mirror: DM495 Nikon, Emission filter: BA500-545 Nikon
Sample
-
Dye
-
Clearing
-
Biological Process
cellular protein localization
(
GO:0034613
)
Cellular Component
-
Project gallery
OME-Zarr endpoint
https://dmss3gw.riken.jp/ssbd/omezarr/v0.4/ssbd-000037/pmPAR-2.zarr/0
Dataset gallery
Sibling records
Quantitative data
BDML file of the PAR-2 trajectories obtained from time-lapse semi-TIRF microscopy images of fluorescent particles of GFP::phospho-mimicPAR-2 in a C. elegans wild-type embryo