Description
The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. Theoretical investigation of the imaging properties and experimental demonstrations show that selective plane activation is beneficial for imaging dense microstructures in cells and cell spheroids. All the image data in this project is already registered at https://doi.org/10.5281/zenodo.10652642.
Funding Information
This work was partially supported by Core Research for Evolutionary Science and Technology by Japan Science and Technology Agency (grant no. JPMJCR15N3 to T.N., JPMJCR1925 and JPMJPF2009 to K. Fujita). This work was also partially supported by a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan (grant no. 18H05410 to T.N.). Part of this work was performed under the Research Program of ‘Dynamic Alliance for Open Innovation Bridging Human, Environment and Materials’ in ‘Network Joint Research Center for Materials and Devices’ to K. Fujita and T.N. R.H. acknowledges support by the Collaborative Research Center SFB 1278 (Poly Target, project C04) funded by the Deutsche Forschungsgemeinschaft and the Leibniz science campus Infecto-Optics, project HotAim 2.0.