SSBD:database

Project

Dataset ID

Fig2_onephoton_AP_SIM

Kind

Image data

Title

Fluorescence images of autophagosomes in living MEFs obtained in SIM mode for single-photon activation

Description

The autophagosomes labeled with the autophagy marker protein LC3B fused to Skylan-NS in living mouse embryonic fibroblasts (MEFs) in structured illumination microscopy (SIM) mode using single-photon activation. Fluorescence images of autophagosomes in living MEFs in SIM mode were captured. Skylan-NS is a photoswitchable green fluorescent protein . LC3B is to function in expansion of the autophagosomal membrane. CW laser is used for single-photon activation, which is more efficient and allows measurement with higher temporal resolution.

Folder Name

Fig2_onephoton_AP_SIM

File Format

tif

File Size

9MB

File Separation Method

-

Figure Number

Figure2l

Dimensions

3069x3069x1x1x1

X Scale

70.9 micrometer

Y Scale

70.9 micrometer

Z Scale

-

T Scale

-

Channel

1 channel

Quantitative Data

-

Description

The Skylan-NS-LC3B was transduced into MEFs and could be expressed stably. Skylan-NS was transduced into MEFs to improve the super-resolution imaging for autogosomes. LC3B was to function in expansion of the autophagosomal membrane.

Organism

Strain

-

Cell

fibroblast (CL:0000057)

Cell line

-

MeSH

Intrinsic Variable

-

Extrinsic Variable

The Skylan-NS-LC3B was transduced into MEFs

Anatomical Entity

-

UBERON

-

Biological Process

-

Cellular Component

Molecular Function

-

MeSH Anatomy

MeSH BP

MeSH CC

MeSH MF

Imaging Method

Detection Method

detection of photons

Visualization Method

Skylan-NS LC3B

Illumination Method

wide-field illumination

Source of Contrast

-

Imaged Parameter

-

Sample Preparation Method

living cell

MeSH

Body

-

Module

-

Light Source

405 nm wide-field illumination

Detector

Hamamatsu Photonics ORCA-Flash 4.0 v3

Objective

Nikon CFI75 Apo 25XC W, Special Optics 54-10-7

Filter Set

-

Dichroic

Di03-R488-t3-25x36, RazorEdge LongPass488