344-Murata-InfectHeart
Author reviewedInfluence of persistent SARS-CoV-2 infection on a three- dimensional cardiac model
Project ID
344-Murata-InfectHeart
Project SID
344
Title
Influence of persistent SARS-CoV-2 infection on a three- dimensional cardiac model
Description
Patients with chronic cardiomyopathy may have persistent viral infections in their hearts, particularly with SARS-CoV-2, which targets the ACE2 receptor highly expressed in human hearts. This raises concerns about a potential global heart failure pandemic stemming from COVID-19, an SARS-CoV-2 pandemic in near future. Although faced with this healthcare caveat, there is limited research on persistent viral heart infections, and no models have been established. In this study, we created an SARS-CoV-2 persistent infection model using human iPS cell-derived cardiac microtissues (CMTs). Mild infections sustained viral presence without signif- icant dysfunction for a month, indicating persistent infection. However, when exposed to hypoxic conditions mimicking ischemic heart diseases, cardiac function deteriorated alongside intracellular SARS-CoV-2 reacti- vation in cardiomyocytes and disrupted vascular network formation. This study demonstrates that SARS- CoV-2 persistently infects the heart opportunistically causing cardiac dysfunction triggered by detrimental stimuli such as ischemia, potentially predicting a post COVID-19 era heart failure pandemic.
Submission Date
2024-04-25
Opened Date
-
Release Date
2024-12-14
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
Clinical Translational Research Program
Total
40 files / 1.3 GB
Image datasets
zipped 10 files / 661.8 MB, raw image 30 files / 665.3 MB, total 40 files / 1.3 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Homo sapiens
(
NCBITaxon:9606
)
Strain
-
Cell
differentiated iPS cells
Cell line
-
MeSH
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Ontology
Anatomical Entity
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UBERON
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Biological Process
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Cellular Component
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Molecular Function
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Medical Subject Headings
-
Imaging Method
Method involved in biological imaging
fluorescence microscopy
(
FBbi:00000246
)
time lapse microscopy
(
FBbi:00000249
)
Paper DOI / Paper URL
People
Contact
Keizo Tomonaga
Kyoto University
LabLaboratory of RNA Viruses, Department of Virus Research, Institute for Life and Medical Sciencesoratory for Developmental Dynamics
-
-
Hidetoshi Masumoto
RIKEN
Clinical Translational Research Program, Center for Biosystems Dynamics Research
-
-
Imaging dataset contributor
Kozue Murata (RIKEN)
Quantitative dataset contributor
-
Image datasets
10
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Immunofluorescence images of cardiac microtissues for cTnT, S protein, and DAPI after 28 days of infection with SARS-CoV-2 in mild titers
Immunofluorescence images of cardiac microtissues for cTnT, S protein, and DAPI after 28 days of mock-infection
Immunofluorescence images for persistent infection model of SARS-CoV-2 after 18h of hypoxia treatment followed by 48h reperfusion treatment
Immunofluorescence images for persistent infection model of SARS-CoV-2 after 18h of normoxia treatment followed by 48h reperfusion treatment
Immunofluorescence images for persistent infection model of SARS-CoV-2 before treatment
Time lapse images of pulsating cardiac microtissues
Time lapse images of pulsating cardiac microtissues without infection of SARS-CoV-2 after hypoxia-reperfusion
Time lapse images of pulsating cardiac microtissues without infection of SARS-CoV-2 before hypoxia-reperfusion
Time lapse images of pulsating cardiac microtissues with persistent infection of SARS-CoV-2 after hypoxia-reperfusion
Time lapse images of pulsating cardiac microtissues with persistent infection of SARS-CoV-2 before hypoxia-reperfusion