344-Murata-InfectHeart/VideoS3_WithInfection_after
Author reviewedTime lapse images of pulsating cardiac microtissues with persistent infection of SARS-CoV-2 after hypoxia-reperfusion
Project
Dataset ID
VideoS3_WithInfection_after
Kind
Image data
Title
Time lapse images of pulsating cardiac microtissues with persistent infection of SARS-CoV-2 after hypoxia-reperfusion
Description
Time lapse images of pulsating cardiac microtissues with persistent infection of SARS-CoV-2 after hypoxia-reperfusion (low-oxygen environment , <0.1% oxygen concentration). the persistently infected the microtissues with SARS-CoV-2 exhibited no increase in pulsating frequency and showed further deterioration of the contractile function.
Folder Name
VideoS3_WithInfection_after
File Format
.mp4
File Size
179MB
File Separation Method
-
Figure Number
VideoS3
Dimensions
-
X Scale
-
Y Scale
-
Z Scale
-
T Scale
0.04 seconds
Channel
1 channel
Quantitative Data
-
Biosample
Description
Human iPS cell-derived cardiac microtissues. They are composed of cardiomyocytes and other cardiac component cells (vascular endothelial cells and vascular mural cells) differentiated from human iPS cells and yield a vascular network-like structure that morphologically and functionally mimics the human heart.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
differentiated iPS cells
Cell line
-
MeSH
-
Intrinsic Variable
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Extrinsic Variable
-
Ontology
Anatomical Entity
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UBERON
-
Biological Process
cardiac muscle contraction (GO:0060048) (GO:0060048)
viral process (GO:0016032)
Cellular Component
-
Molecular Function
-
MeSH Anatomy
-
MeSH BP
-
MeSH CC
-
MeSH MF
-
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
-
Treatments
Reagent / Compound
SARS-CoV-2/UT-NCGM02/Human/2020/Tokyo
Concentration
MOI-30
Fold Dilution
-
Imaging Method
Imaging Method
time lapse microscopy (FBbi:00000249)
Detection Method
wide-field detection
Visualization Method
-
Illumination Method
widefield illumination
Source of Contrast
differences in amount of elastic light scattering
Imaged Parameter
-
Sample Preparation Method
living tissue
MeSH
-
Instrument
Body
Nikon ECLIDSE Ts2R
Module
-
Light Source
-
Detector
-
Objective
Nikon CFI 10X
Filter Set
-
Dichroic
-