393-Fujimoto-CoV2Infect/Fig5C_VB_SARS
Author reviewedZ-series images of actin filaments in WT-VB in the SARS-CoV-2-infected device culturing KO-BO
Project
Dataset ID
Fig5C_VB_SARS
Kind
Image data
Title
Z-series images of actin filaments in WT-VB in the SARS-CoV-2-infected device culturing KO-BO
Description
Z-series images of vascular bed in the SARS-CoV-2-infected device in which vascular bed and KO-BO were co-culured. KO-BO is bronchial organoids in which Interferon beta (IFNB1) was knocked out.
Channel1: DAPI, Channel2: Alexa Fluor 647 labeled phalloidin, Channel3: GFP, Channel4: ICAM1, Channel5: Differential interference image
Folder Name
Fig5C_VB_SARS
File Format
.tif
File Size
18.7MB
File Separation Method
-
Figure Number
Fig5C
Dimensions
248x250x30x5x1
X Scale
1.24 micrometer
Y Scale
1.24 micrometer
Z Scale
4.88 micrometer
T Scale
-
Channel
5 channel
Quantitative Data
-
Biosample
Description
Co-culture of bronchial organoids, which were derived from NHBEs (normal human bronchial epithelial
cells), and vascular beds, which were composed from GFP-Expressing Human Umbilical Vein Endothelial Cells, in a special microfluidic device. Interferon beta (IFNB1) was knocked out in the NHBEs.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
endothelial cell of umbilical vein,
bronchial epithelial cell (CL:0002328)
Cell line
-
MeSH
Intrinsic Variable
Interferon beta (IFNB1) was knocked out in NHBEs which is the component of bronchial organoids.
Extrinsic Variable
The SARS-CoV-2 virus was applied to the bronchial organoids,
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
viral process (GO:0016032)
Cellular Component
nucleus (GO:0005634)
actin cytoskeleton (GO:0015629)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
-
Gene
-
Protein / Probe
Intercellular adhesion molecule 1 / antibody
Method / Genotype
-
Gene
-
Protein / Probe
Actin, cytoplasmic 1 / affinity for specific proteins,
phalloidin
Method / Genotype
-
Gene
-
Protein / Probe
-DAPI
Method / Genotype
-
Gene
IFNB1
Protein / Probe
-
Method / Genotype
-
Treatments
Reagent / Compound
SARS-CoV-2 virus
Concentration
0.1 multiplicity of infection
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
primary antibody plus labeled secondary antibody,
GFP,Fig4F
4',6-diamidino-2-phenylindole (DAPI)
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method
MeSH
Instrument
Body
Olympus-Evident FV3000
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident UPLXAPO10X
Filter Set
-
Dichroic
-