SSBD:database v3

393-Fujimoto-CoV2Infect

Project

Title

SARS-CoV-2 infection to the co-culture of bronchial organoids and vascular beds in a microfluidic device

Description

Blood vessels show various COVID-19-related conditions including thrombosis and cytokine propagation. Existing in vitro blood vessel models cannot represent the consequent changes in the vascular structure or determine the initial infection site, making it difficult to evaluate how epithelial and endothelial tissues are damaged. Here, the authors developed a microphysiological system (MPS) that co-culture the bronchial organoids and the vascular bed to analyze infection site and interactions. In this system, virus-infected organoids caused damage in vascular structure. However, vasculature was not damaged or infected when the virus was directly introduced to vascular bed. The knockout of interferon-related genes and inhibition of the JAK/STAT pathway reduced the vascular damage, indicating the protective effect of interferon response suppression. The results demonstrate selective infection of bronchial epithelial cells and vascular damage by cytokines and also indicate the applicability of MPS to investigate how the infection influences vascular structure and functions.

License

CC BY

Released at

Oct. 20, 2025, midnight

Updated at

Funding

KAKENHI (Grant-in-Aid for Scientific Research) on Priority Areas ‘Systems Genomics’ [17017038]

# of image datasets

9

Papers

Papers

Paper information: Fujimoto K, Kameda Y, Nagano Y, Deguchi S, Yamamoto T, Krol RP, Gee P, Matsumura Y, Okamoto T, Nagao M, Takayama K, Yokokawa R. SARS-CoV-2-induced disruption of a vascular bed in a microphysiological system caused by type-I interferon from bronchial organoids. Lab Chip . 2024 Aug 6;24(16):3863-3879.,
Pubmed ID: 38252025,
DOI: https://doi.org/0.1039/d3lc00768e,
URL: https://pubmed.ncbi.nlm.nih.gov/38252025/

Contacts

Contacts

Ryuji Yokokawa (Contact) , Kyoto University
Kazuo Takayama (Contact) , Kyoto University
Kazuya Fujimoto (Imaging contributor) , Kyoto University
Yoshikazu Kameda (Imaging contributor) , Kyoto University
Yuta Nagano (Imaging contributor) , Kyoto University

Biosamples

Organisms

Homo sapiens (NCBI:txid9606)

Strains

Cells

endothelial cell of umbilical vein (CL_0002618)
bronchial epithelial cell (CL_0002328)

Cell lines

Ontologies

UBERON