393-Fujimoto-CoV2Infect
Author reviewedSARS-CoV-2 infection to the co-culture of bronchial organoids and vascular beds in a microfluidic device
Project ID
393-Fujimoto-CoV2Infect
Project SID
393
Title
SARS-CoV-2 infection to the co-culture of bronchial organoids and vascular beds in a microfluidic device
Description
Blood vessels show various COVID-19-related conditions including thrombosis and cytokine propagation. Existing in vitro blood vessel models cannot represent the consequent changes in the vascular structure or determine the initial infection site, making it difficult to evaluate how epithelial and endothelial tissues are damaged. Here, the authors developed a microphysiological system (MPS) that co-culture the bronchial organoids and the vascular bed to analyze infection site and interactions. In this system, virus-infected organoids caused damage in vascular structure. However, vasculature was not damaged or infected when the virus was directly introduced to vascular bed. The knockout of interferon-related genes and inhibition of the JAK/STAT pathway reduced the vascular damage, indicating the protective effect of interferon response suppression. The results demonstrate selective infection of bronchial epithelial cells and vascular damage by cytokines and also indicate the applicability of MPS to investigate how the infection influences vascular structure and functions.
Submission Date
2024-10-31
Opened Date
-
Release Date
2025-11-26
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
KAKENHI (Grant-in-Aid for Scientific Research) on Priority Areas ‘Systems Genomics’ [17017038]
Total
487 files / 14.4 GB
Image datasets
zipped 9 files / 1.2 GB, raw image 44 files / 2.0 GB, total 53 files / 3.2 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Homo sapiens
(
NCBITaxon:9606
)
Strain
-
Cell
endothelial cell of umbilical vein,
bronchial epithelial cell
(
CL:0002328
)
Cell line
-
MeSH
Ontology
Anatomical Entity
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UBERON
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Biological Process
viral process
(
GO:0016032
)
Cellular Component
nucleus,
actin cytoskeleton
(
GO:0015629
)
actin cytoskeleton
(
GO:0015629
)
Molecular Function
-
Medical Subject Headings
Imaging Method
Method involved in biological imaging
fluorescence microscopy
(
FBbi:00000246
)
confocal microscopy
(
FBbi:00000251
)
Paper DOI / Paper URL
People
Contact
Ryuji Yokokawa
Kyoto University
-
-
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Kazuo Takayama
Kyoto University
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Imaging dataset contributor
Kazuya Fujimoto (Kyoto University)
Yoshikazu Kameda (Kyoto University)
Yuta Nagano (Kyoto University)
Quantitative dataset contributor
-
Image datasets
9
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Fluorescent images of the vascular bed 4days-after infection with mock virus
Fluorescent images of the vascular bed 4days-after infection with SARS-CoV-2 virus
Fluorescent images of the vascular bed before infection with mock virus or SARS-CoV-2 virus
Z-series images of the vascular bed after infection with mock virus
Z-series images of the vascular bed after infection with SARS-CoV-2 virus
Z-series images of KO-VB in the SARS-CoV-2-infected device
Z-series images of actin filaments in WT-VB in the SARS-CoV-2-infected device culturing KO-BO
Z-series images of actin filaments in VB in SARS-CoV-2-infected devices with the administration of baricitinib
Z-series images of actin filaments in VB in SARS-CoV-2-infected devices with the administration of tofacitinib