393-Fujimoto-CoV2Infect/Fig4F_VB_Mock
Author reviewedZ-series images of the vascular bed after infection with mock virus
Project
Dataset ID
Fig4F_VB_Mock
Kind
Image data
Title
Z-series images of the vascular bed after infection with mock virus
Description
Z-series images of the vascular bed after infection with mock virus. Bronchial organoids and vascular bed composed of HUVECs (Human Umbilical Vein Endothelial Cells) were co-cultured in a special 3D microfluidic device. The virus was applied to the side of bronchial organoids, but not to the vascular bed side.
Channel1: ICAM1, Channel2: Alexa Fluor 647 labeled phalloidin, Channel3: DAPI, Channel4: GFP, Channel4: Differential interference image
Folder Name
Fig4F_VB_Mock
File Format
.oir
File Size
661MB
File Separation Method
-
Figure Number
Fig4F
Dimensions
2002x2972x11x5x1
X Scale
1.24 micrometer
Y Scale
1.24 micrometer
Z Scale
5.18 micrometer
T Scale
-
Channel
5 channel
Quantitative Data
-
Biosample
Description
Co-culture of bronchial organoids, which were derived from normal human bronchial epithelial
cells, and vascular beds, which were composed from GFP-Expressing Human Umbilical Vein Endothelial Cells, in a special microfluidic device.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
endothelial cell of umbilical vein,
bronchial epithelial cell (CL:0002328)
Cell line
-
MeSH
Intrinsic Variable
The commercial HUVECSs expressing GFP were used.
GFP-HUVECs
(Angio-Proteomie, cAP-0001, Boston, MA, USA)
Extrinsic Variable
The mock virus was applied to the bronchial organoids,
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
viral process (GO:0016032)
Cellular Component
nucleus (GO:0005634)
actin cytoskeleton (GO:0015629)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
-
Gene
-
Protein / Probe
Intercellular adhesion molecule 1 / antibody
Method / Genotype
-
Gene
-
Protein / Probe
Actin, cytoplasmic 1 / affinity for specific proteins,
phalloidin
Method / Genotype
-
Gene
-
Protein / Probe
-DAPI
Method / Genotype
-
Treatments
Reagent / Compound
SARS-CoV-2 virus
Concentration
0.1 multiplicity of infection
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
primary antibody plus labeled secondary antibody,
GFP,
4',6-diamidino-2-phenylindole (DAPI)
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method
MeSH
Instrument
Body
Olympus-Evident FV3000
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident UPLXAPO10X
Filter Set
-
Dichroic
-