387-Ando-StayGold/VIdeo7_Ftractin_2
Author reviewedTime-lapse images of F-tractin=mStayGold in COS-7 cells
Project
Dataset ID
VIdeo7_Ftractin_2
Kind
Image data
Title
Time-lapse images of F-tractin=mStayGold in COS-7 cells
Description
Time-lapse images of F-tractin=mStayGold, which visualize filamentous actin, in COS-7 cells.
Folder Name
VIdeo7_Ftractin_2
File Format
.tif
File Size
573MB
File Separation Method
-
Figure Number
Video7
Dimensions
548x548x1x1x1500
X Scale
0.04 micrometer
Y Scale
0.04 micrometer
Z Scale
-
T Scale
0.31 second,
3.18 frames/second
Channel
1 channel
Quantitative Data
-
Biosample
Description
COS-7 cells expressing F-tractin=mStayGold
Organism
Chlorocebus aethiops (NCBITaxon:9534)
Strain
-
Cell
-
Cell line
COS-7 cell (CLO:0002597)
Intrinsic Variable
-
Extrinsic Variable
F-tractin=mStayGold was transfected
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
actin cytoskeleton (GO:0015629)
Molecular Function
-
MeSH Anatomy
Genetics
Gene
-
Protein / Probe
-Ftractin, a probe for filamentous actin
Method / Genotype
transfection
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
spinning disk confocal microscopy (FBbi:00000253)
single-beam laser-scanning
confocal microscopy (LSCM)
Detection Method
-
Visualization Method
mStayGold, a StayGold derivative
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
Instrument
Body
Olympus-Evident IX83
Module
-
Light Source
-
Detector
Hamamatsu ORCA-Flash4.0 CL, USB
Objective
Olympus-Evident UPLAPO100XOHR
Filter Set
-
Dichroic
-