387-Ando-StayGold
Author reviewedSuper resolution cellular images using fluorescent-tags of StayGold variants
Project ID
387-Ando-StayGold
Project SID
387
Title
Super resolution cellular images using fluorescent-tags of StayGold variants
Description
Although StayGold is a bright and highly photostable fluorescent protein, its propensity for obligate dimer formation may hinder applications in molecular fusion and membrane targeting. To attain monovalent as well as bright and photostable labeling, the authors engineered tandem dimers of StayGold to promote dispersibility. On the basis of the crystal structure of this fluorescent protein, they disrupted the dimerization to generate a monomeric variant that offers improved photostability and brightness compared to StayGold. The authors applied the new monovalent StayGold tools to live-cell imaging experiments using spinning-disk laser-scanning confocal microscopy or structured illumination microscopy. They achieved cell-wide, high-spatiotemporal resolution and sustained imaging of dynamic subcellular events, including the targeting of endogenous condensin I
to mitotic chromosomes, the movement of the Golgi apparatus and its membranous derivatives along microtubule networks, the distribution of cortical filamentous actin and the remolding of cristae membranes within mobile mitochondria.
Submission Date
2024-11-14
Opened Date
-
Release Date
2025-11-26
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
This work was supported in part by Grant-in-Aid for Scientific Research (S) (21H05041 to A.M.), Grant-in-Aid for Innovative Areas: Resonance Bio (15H05948 to A.M.) and Information Physics of
Living Matters (19H05794 and 19H05795 to Y.O.), Japan Science and Technology Agency Core Research for Evolutionary Science and Technology program, ‘Spatiotemporal dynamics of intracellular components’ (JPM JCR20E2 to Y.O.) and Moonshot R&D program ‘Realization of ultra-early disease prediction and intervention by
2050’ (JPM JMS2025-14 to Y.O.), Marine Biomass Innovation Project (NFRFT-2020-00452 to A.M.), the Brain Mapping by Integrated Neurotechnologies for Disease Studies from AMED (Brain/MINDS, JP15dm0207001 to A.M.), RIKEN Collaboration Seed Fund (to M.T. and R.A.) and Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED (JP21am0101070 to M.Y.).
Total
1432 files / 710.0 GB
Image datasets
zipped 31 files / 73.0 GB, raw image 265 files / 153.6 GB, total 296 files / 226.6 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Homo sapiens
(
NCBITaxon:9606
)
Chlorocebus aethiops
(
NCBITaxon:9534
)
Strain
-
Cell
-
Cell line
MeSH
Ontology
Anatomical Entity
-
UBERON
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Biological Process
chromosome organization
(
GO:0051276
)
negative regulation of actin filament polymerization
(
GO:0030837
)
cellular response to histamine,
cellular response to ionomycin
(
GO:1904637
)
Golgi organization
(
GO:0007030
)
Cellular Component
nucleus,
chromosome
(
GO:0005694
)
Golgi apparatus
(
GO:0005794
)
actin cytoskeleton
(
GO:0015629
)
mitochondrion
(
GO:0005739
)
Molecular Function
regulate the chromosome assembly and segregation
Medical Subject Headings
Imaging Method
Method involved in biological imaging
spinning disk confocal microscopy
(
FBbi:00000253
)
single-beam laser-scanning
confocal microscopy (LSCM)
structured illumination microscopy (SIM) (Fbbi:,00000332)
lattice SIM
Paper DOI / Paper URL
People
Contact
Atsushi Miyawaki
RIKEN
Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
-
-
Imaging dataset contributor
Ryoko Ando (RIKEN)
Satoshi Shimozono (RIKEN)
Masatoshi Takagi (RIKEN)
Mayu Sugiyama (RIKEN)
Hiroshi Kurokawa (RIKEN)
Fumiyoshi Ishidate (Kyoto University)
Takahiro Fujiwara (Kyoto University)
Yasushi Okada (RIKEN)
Quantitative dataset contributor
-
Image datasets
31
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse images of CAP-H-td5oxStayGold and SiR-DNA-labeled chromosomes in HCT116 cells
Time-lapse images of CAP-H-mClover3 in HCT116 cells during prometaphase
Time-lapse images of CAP-H-td5oxStayGold in HCT116 cells during prometaphase
Z-series images of td5StayGold(c4)=GianCreg and GM130 in fixed HeLa cells
Z-series images of td5StayGold(c4)=GianCreg and GM130 in fixed HeLa cells
Z-series images of td5StayGold(c4)=GianCreg and GM130 in fixed HeLa cells
Z-series images of td5StayGold(c4)=GianCreg and Giantin in fixed HeLa cells
Z-series images of td5StayGold(c4)=GianCreg and Giantin in fixed HeLa cells
Z-series images of td5StayGold(c4)=GianCreg and Giantin in fixed HeLa cells
Z-series images of td5StayGold(c4)=GianCreg and TGN46 in fixed HeLa cells
Z-series images of td5StayGold(c4)=GianCreg and TGN46 in fixed HeLa cells
Z-series images of td5StayGold(c4)=GianCreg and TGN46 in fixed HeLa cells
Time-lapse images of F-tractin=mStayGold in COS-7 cells treated with cytochalasin D
Time-lapse images of F-tractin=mStayGold in COS-7 cells treated with latrunculin A.
Time-lapse images of mStayGld(c4)=UtrCH in COS-7 cells treated with cytochalasin D
Time-lapse images of mStayGld(c4)=UtrCH in COS-7 cells treated with latrunculin A.
Time-lapse images of CAP-H-td5oxStayGold and SiR-DNA-labeled chromosomes in HCT116 cells
Time-lapse lattice SIM images of COX8a=mStayGold in HeLa cells
Time-lapse images of td5StayGold(c4)=GianCreg and td8ox2StayGold(c4)=beta-tubulin in COS-7 cells by dual-target imaging
Time-lapse images of F-tractin=mStayGold in COS-7 cells
Time-lapse images of F-tractin=mStayGold in COS-7 cells
Time-lapse images of COX8a=mStayGold in HeLa cells, showing relatively stable mitochondrial membrane
Time-lapse images of COX8a=mStayGold in HeLa cells, showing extremely dynamic and complicated mitochondrial membrane
Time-lapse images of COX8a=mStayGold in HeLa cells in series application of histamine, cyproheptadine, and ionomycin.
Time-lapse images of COX8a=mStayGold in HeLa cells in series application of histamine, cyproheptadine, and ionomycin.
Time-lapse and z-series images of CAP-H-td5oxStayGold and SiR-DNA-labeled chromosomes in HCT116 cells
Time-lapse and Z-series images of td5StayGold(c4)=GianCreg in HeLa cells
Time-lapse and Z-series images of td5StayGold(c4)=GianCreg in HeLa cells
Time-lapse and Z-series images of td5StayGold(c4)=GianCreg and td8ox2StayGold(c4)=beta-tubulin in COS-7 cells by dual-target imaging
Time-lapse images of mStayGold(c4)=UtrCH in COS-7 cells
Time-lapse images of mStayGold(c4)=UtrCH in COS-7 cells