371-Liao-RamanSpectra/Fig4_HepG2FFA
Author reviewedRaman spectrua data of HepG2 spheroids treated with various concentrations of FFA
Project
Dataset ID
Fig4_HepG2FFA
Kind
Image data
Title
Raman spectrua data of HepG2 spheroids treated with various concentrations of FFA
Description
Raman spectra data of HepG2 spheroids treated with various concentrations of FFA (free fatty acid: 0 mM, 0.01 mM, 0.1 mM, 0.25 mM, 0.5 mM, 1.0 mM, 2.0 mM). Multiple spectra data is deposited for each concentration of FFA.
Folder Name
Fig4_HepG2FFA
File Format
.xlsx
File Size
1.1MB
File Separation Method
-
Figure Number
Fig4
Dimensions
-
X Scale
-
Y Scale
-
Z Scale
-
T Scale
-
Channel
-
Quantitative Data
-
Biosample
Description
HepG2 spheroids
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
-
Cell line
Hep G2 cell (CLO:0003704)
Intrinsic Variable
-
Extrinsic Variable
Treatment with several concentrations of free fatty acid
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
-
Molecular Function
-
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
-
Treatments
Reagent / Compound
free fatty acid
Concentration
0.01, 0.1, 0.25, 0.5, 1.0, 2.0 millimolar
Fold Dilution
-
Imaging Method
Imaging Method
inelastic scattering of photons (Raman scattering) (FBbi:00000589)
Detection Method
charge coupled device (CCD)
Visualization Method
Intensity of Raman Scattering
Illumination Method
array illumination
Source of Contrast
differences in amount of elastic light scattering
Imaged Parameter
-
Sample Preparation Method
living spheroid cells
Instrument
Body
home-built device
Module
home-built device
Light Source
CW laser
Detector
2D cooled CCD camera (PIXIS 400B eXcelon, Teledyne Princeton Instruments)
Objective
spherical lens
Filter Set
Custom-made filter
Dichroic
Custom-made filter