SSBD:database

Project ID

371-Liao-RamanSpectra

Project SID

371

Title

Raman spectra data of cell spheroids on a multi-well plate treated with various concentrations of reagents

Description

Cell spheroids offer alternative in vitro cell models to monolayer cultured cells because they express complexities similar to thoseof in vivo tissues, such as cellular responses to drugs and chemicals. Raman spectroscopy emerged as a powerful analytical tool for detecting chemical changes in living cells because it nondestructively provides vibrational information regarding a target. Although multiple iterations are required in drug screening to determine drugs to treat cell spheroids and assess the interspheroid heterogeneity, current Raman applications used in spheroidsanalysis allow the observation of only a few spheroids owing to the lowthroughput of Raman spectroscopy. In this study, the authors developed a multifocal Raman spectrophotometer that enables simultaneous analysis of multiple spheroids in separate wells of a regular 96-well plate. By utilizing 96 focal spots excitation and parallel signal collection, their system can improve the throughput by approximately 2 orders of magnitude compared to a conventional single-focus Raman microscope. The Raman spectra of HeLa cell spheroids treated with anticancer drugs and HepG2 cell spheroids treated with free fatty acids were measured simultaneously, and concentration-dependent cellular responses were observed in both studies. Using the multifocal Raman spectrophotometer, they rapidly observed chemical changes in spheroids, and thus, this system can facilitate the application of Raman spectroscopy in analyzing the cellular responses of spheroids.

Submission Date

2024-09-02

Opened Date

-

Release Date

2025-01-31

Update Date

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Kind

License

Project URL

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Project DOI

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Metadata Version

-

Template Version

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Funding Information

This research was supported by Osaka University Innovation Bridge Grant and JST COI-NEXT under grant number JPMJPF2009.

Total

4 files / 2.6 MB

Image datasets

zipped 2 files / 1.3 MB, raw image 2 files / 1.3 MB, total 4 files / 2.6 MB

Quantitative datasets

zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Organism

Strain

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Cell

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Cell line

MeSH

Anatomical Entity

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UBERON

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Biological Process

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Cellular Component

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Molecular Function

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Medical Subject Headings

Method involved in biological imaging