371-Liao-RamanSpectra/Fig3_HeLaActD
Author reviewedRaman spectra data of HeLa spheroids treated with various concentrations of actinomycin D
Project
Dataset ID
Fig3_HeLaActD
Kind
Image data
Title
Raman spectra data of HeLa spheroids treated with various concentrations of actinomycin D
Description
Raman spectra data of HeLa spheroids treated with various concentrations of actinomycin D (0 uM, 0.1 uM, 0.3 uM, 1 uM, 3 uM, 10 uM). Multiple spectra data is deposited for each concentration of actinomycin D.
Folder Name
Fig3_HeLaActD
File Format
.xlsx
File Size
241KB
File Separation Method
-
Figure Number
Fig3
Dimensions
-
X Scale
-
Y Scale
-
Z Scale
-
T Scale
-
Channel
-
Quantitative Data
-
Biosample
Description
HeLa spheroids of 250−300 micrometer diameters
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
-
Cell line
HeLa Cell (CLO:0003684)
Intrinsic Variable
-
Extrinsic Variable
Treatment with several concentrations of actinomycin D
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
-
Molecular Function
-
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
-
Treatments
Reagent / Compound
actinomycin D
Concentration
0, 0.1, 0.3, 1.0, 3.0, 10 micromolar
Fold Dilution
-
Imaging Method
Imaging Method
inelastic scattering of photons (Raman scattering) (FBbi:00000589)
Detection Method
charge coupled device (CCD)
Visualization Method
Intensity of Raman Scattering
Illumination Method
array illumination
Source of Contrast
differences in amount of elastic light scattering
Imaged Parameter
-
Sample Preparation Method
living spheroid cells
Instrument
Body
home-built device
Module
home-built device
Light Source
CW laser
Detector
2D cooled CCD camera (PIXIS 400B eXcelon, Teledyne Princeton Instruments)
Objective
spherical lens
Filter Set
Custom-made filter
Dichroic
Custom-made filter