337-Isoe-BrainRegion/Video2_pallium
Author reviewedZ-stack images of medaka brain that visualize clonally related units in the pallium
Project
Dataset ID
Video2_pallium
Kind
Image data
Title
Z-stack images of medaka brain that visualize clonally related units in the pallium
Description
Z-series images of medaka brain obtained by light sheet microscopy that visualize clonally related units in the pallium. This transgenic line express fluorescent protein under HuC promoter, which drives expression in neural progenitor cells. As the reporter GFP was expressed in neural progenitor cells at the neurula stage (stage 16–17) by Cre-loxp recombination system, we can trace the lineage in the adult fish. GFP-positive cells (channel2) make a cluster and send a projection to the same site. Red signals indicate DsRed (channel1) and blue signals indicate DAPI staining (channel3). Most of the GFP-positive cells in the pallium are not mixed with DsRed-positive cells and did not locate across multiple regions. In this brain, the clonally related units are detected in Dld and Dd2 region of the pallium.
Folder Name
Video2_pallium
File Format
.tif
File Size
347MB
File Separation Method
-
Figure Number
Figure1CD
Dimensions
362x324x493x3x1
X Scale
-
Y Scale
-
Z Scale
-
T Scale
-
Channel
3 channel
Quantitative Data
-
Biosample
Description
The medaka brain deriverd from two transgenic lines. One is the transgenic line (Tg (HuC:loxp-DsRed-loxp-GFP)) that visualized neural progenitors by the HuC promoter. The second is a line (Tg (HSP-Cre)) that expresses Cre recombinase under the heat shock protein promoter. In the absence of the recombination, DsRed is expressed in the neural progenitor cells. When Cre-loxp recombination is induced the neural progenitors express GFP instead of DsRed.
Organism
Oryzias latipes (NCBITaxon:8090)
Strain
HuC:loxp-DsRed-loxp-GFP, HSP-Cre
Cell
neuron (CL:0000541)
Cell line
-
MeSH
Neurogenesis (D055495)
Intrinsic Variable
Two transgenic medaka lines. One is the transgenic line (Tg (HuC:loxp-DsRed-loxp-GFP)) that visualized neural progenitors by the HuC promoter. The second is a line (Tg (HSP-Cre)) that expresses Cre recombinase under the heat shock protein promoter.
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
brain (UBERON:0000955)
Biological Process
neurogenesis (GO:0022008) (GO:0022008)
neuron migration (GO:0001764)
Cellular Component
-
Molecular Function
-
MeSH Anatomy
Neurogenesis (D055495)
MeSH BP
Neurogenesis (D055495)
MeSH CC
Neurogenesis (D055495)
MeSH MF
Neurogenesis (D055495)
Genetics
Gene
-
Protein / Probe
-DAPI
Method / Genotype
transgenic / HuC:loxp-DsRed-loxp-GFP, HSP-Cre
Gene
-
Protein / Probe
-DAPI
Method / Genotype
transgenic / HuC:loxp-DsRed-loxp-GFP, HSP-Cre
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
light-sheet microscopy
Detection Method
charge coupled device (CCD)
Visualization Method
expression of fluorescient protein & DAPI staining
Illumination Method
light-sheet illumination (FBbi:00000364)
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
whole mounted tissue
MeSH
Neurogenesis (D055495)
Instrument
Body
custom made
Module
custom made
Light Source
Argon-krypton laser
Detector
-
Objective
Zeiss Achroplan 20x/0.6
Filter Set
RazorEdge RU 488, 568, Semrock
Dichroic
-