337-Isoe-BrainRegion/Video1_ReferenceBrain
Author reviewedZ-stack images of horizontal section of the reference medaka brain stained with DAPI
Project
Dataset ID
Video1_ReferenceBrain
Kind
Image data
Title
Z-stack images of horizontal section of the reference medaka brain stained with DAPI
Description
Z-series images of horizontal section of the reference medaka brain obtained by light sheet microscopy. The authors observed three-dimensional distribution of cell nuclei by DAPI staining and tissue clearing, and redefined the anatomical regions in the adult medaka (2-month-old) telencephalon.
Folder Name
Video1_ReferenceBrain
File Format
.tif
File Size
24MB
File Separation Method
-
Figure Number
Figure1A
Dimensions
300x250x319x1x1
X Scale
-
Y Scale
-
Z Scale
-
T Scale
-
Channel
1 channel
Quantitative Data
-
Biosample
Description
The wild type medaka brain.
Organism
Oryzias latipes (NCBITaxon:8090)
Strain
-
Cell
neuron (CL:0000540)
Cell line
-
MeSH
-
Intrinsic Variable
-
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
brain (UBERON:0000955)
Biological Process
-
Cellular Component
-
Molecular Function
-
MeSH Anatomy
-
MeSH BP
-
MeSH CC
-
MeSH MF
-
Genetics
Gene
-
Protein / Probe
-DAPI
Method / Genotype
-
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
light-sheet microscopy
Detection Method
charge coupled device (CCD)
Visualization Method
DAPI staining
Illumination Method
light-sheet illumination (FBbi:00000364)
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
whole mounted tissue
MeSH
-
Instrument
Body
custom made
Module
custom made
Light Source
Argon-krypton laser
Detector
-
Objective
Zeiss Achroplan 20x/0.5
Filter Set
RazorEdge RU 488, 568, Semrock
Dichroic
-