SSBD:database

Project

Dataset ID

Brain_21

Kind

Image data

Title

Z-stack images of horizontal section of medaka telencephalon visualizing units of GFP-positive cells, each of which has the same lineage

Description

Z-series images of medaka brain obtained by light sheet microscopy that visualize clonally related units in the pallium. This transgenic line express fluorescent protein under HuC promoter, which drives expression in neural progenitor cells. As the reporter GFP was expressed in neural progenitor cells at the neurula stage (stage 16–17) by Cre-loxp recombination system, we can trace the lineage in the adult fish. We can see some GFP-positive clonally related compartments that distribute exclusively in one anatomical region but not across many anatomical regions. DsRed (channel1), GFP (channel2), and DAPI staining (channel3)

Folder Name

Brain_21

File Format

.tif

File Size

110MB

File Separation Method

-

Figure Number

Figure1D

Dimensions

362x324x312x3x1

X Scale

-

Y Scale

-

Z Scale

-

T Scale

-

Channel

3 channel

Quantitative Data

-

Description

The medaka brain deriverd from two transgenic lines. One is the transgenic line (Tg (HuC:loxp-DsRed-loxp-GFP)) that visualized neural progenitors by the HuC promoter. The second is a line (Tg (HSP-Cre)) that expresses Cre recombinase under the heat shock protein promoter. In the absence of the recombination, DsRed is expressed in the neural progenitor cells. When Cre-loxp recombination is induced the neural progenitors express GFP instead of DsRed.

Organism

Oryzias latipes (NCBITaxon:8091)

Strain

HuC:loxp-DsRed-loxp-GFP, HSP-Cre

Cell

neuron (CL:0000541)

Cell line

-

MeSH

-

Intrinsic Variable

Two transgenic medaka lines. One is the transgenic line (Tg (HuC:loxp-DsRed-loxp-GFP)) that visualized neural progenitors by the HuC promoter. The second is a line (Tg (HSP-Cre)) that expresses Cre recombinase under the heat shock protein promoter.

Extrinsic Variable

-

Anatomical Entity

-

UBERON

brain (UBERON:0000955)

Biological Process

neurogenesis (GO:0022008), neuron migration (GO:0001764)

Cellular Component

-

Molecular Function

-

MeSH Anatomy

-

MeSH BP

Neurogenesis

MeSH CC

-

MeSH MF

-

Imaging Method

light-sheet microscopy

Detection Method

charge coupled device (CCD)

Visualization Method

expression of fluorescient protein & DAPI staining

Illumination Method

light-sheet illumination (FBbi:00000364)

Source of Contrast

distribution of a specific protein

Imaged Parameter

fluorescence emission

Sample Preparation Method

whole mounted tissue

MeSH

-

Body

custom made

Module

custom made

Light Source

Argon-krypton laser

Detector

-

Objective

Zeiss Achroplan 20x/0.26

Filter Set

RazorEdge RU 488, 568, Semrock

Dichroic

-