320-Spratt-dMetRaman/Fig5BC_process
Author reviewedThe processed Raman images of dMet and background signals in TorTED-Drosophila larval fat body
Project
Dataset ID
Fig5BC_process
Kind
Image data
Title
The processed Raman images of dMet and background signals in TorTED-Drosophila larval fat body
Description
The processed Raman images of deuterated methionine (dMet) and background signals in TorTED-Drosophila larval fat body. XXX_Met.jpg is dMet image, and XXX_XPM.jpg is background image. XXX_Met.jpg was genarated from 1.tif and 2.tif which was subtracted 0.tif and 3.tif background signals. XXX_XPM.jpg was generated from 0.tif and 3.tif background signals. The images of GFP fluorescence in the same area is also reposited.
Folder Name
Fig5BC_process
File Format
.jpg
File Size
111KB
File Separation Method
XXX_Met.jpg is dMet image, XXX_GFP.jpg is GFP image, and XXX_XPM.jpg is background image.
Figure Number
Fig5BC
Dimensions
500x500x1x3x1
X Scale
80 micrometer
Y Scale
80 micrometer
Z Scale
-
T Scale
-
Channel
3 channel
Quantitative Data
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Biosample
Description
The dissected fat body from the third instar larvae of Drosophila which was incorporated deuterated methionine (dMet).
Organism
Drosophila (NCBITaxon:7215)
Strain
-
Cell
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Cell line
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MeSH
Fat Body (D005216)
Intrinsic Variable
GFP was expressed in the cells where a dominant negative form of mTOR was expressed.
Extrinsic Variable
Flies were transferred to a modified version of holidic medium supplemented with deuterated methionine (Cambridge Isotope Laboratories: DLM-6797–0.1) instead of methionine, for up to 48 h prior to dissection.
Ontology
Anatomical Entity
embryonic/larval fat body (FBbt:00001704)
UBERON
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Biological Process
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Cellular Component
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Molecular Function
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MeSH Anatomy
Fat Body (D005216)
MeSH BP
Fat Body (D005216)
MeSH CC
Fat Body (D005216)
MeSH MF
Fat Body (D005216)
Genetics
Gene
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Protein / Probe
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Method / Genotype
transgenic / hsFLP, UAS-mCD8-GFP; act5C-FRTstopFRT-GAL4, UAS-GFP, UAS-TorTED
Gene
Tor
Protein / Probe
-
Method / Genotype
transgenic / hsFLP, UAS-mCD8-GFP; act5C-FRTstopFRT-GAL4, UAS-GFP, UAS-TorTED
Treatments
Reagent / Compound
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Concentration
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Fold Dilution
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Imaging Method
Imaging Method
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Detection Method
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Visualization Method
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Illumination Method
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Source of Contrast
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Imaged Parameter
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Sample Preparation Method
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MeSH
Fat Body (D005216)
Instrument
Body
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Module
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Light Source
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Detector
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Objective
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Filter Set
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Dichroic
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