320-Spratt-dMetRaman
Author reviewedStimulated Raman images of deuterated methionine incorporated into adult and larval Drosophila
Project ID
320-Spratt-dMetRaman
Project SID
320
Title
Stimulated Raman images of deuterated methionine incorporated into adult and larval Drosophila
Description
Introduction: Visualizing small individual biomolecules at subcellular resolution in live cells and tissues can provide valuable insights into metabolic activity in heterogeneous cells, but is challenging. Methods: Here, the authors used stimulated Raman scattering (SRS) microscopy to image deuterated methionine (d-Met) incorporated into Drosophila tissues in vivo. Results: Their results demonstrate that SRS can detect a range of previously uncharacterized cell-to-cell differences in d-Met distribution within a tissue at the subcellular level. Discussion: These results demonstrate the potential of SRS microscopy for metabolic imaging of less abundant but important amino acids such as methionine in tissue.
Submission Date
2024-09-21
Opened Date
-
Release Date
2025-04-10
Update Date
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Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
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Template Version
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Funding Information
This work was supported in part by Japan Society for the Promotion of Science KAKENHI under JP20H02650, JP20H05725, JP20H05726, and JP21J00452; in part by Japan Science and Technology Agency Core Research for Evolutional Science and Technology (CREST) under Grant JPMJCR 1872; in part by Nakatani Foundation Grant for Technology Development Research, and in part by Quantum Leap Flagship Program of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) under Grant JPMXS0118067246.
Biosample
Organism
Drosophila
(
NCBITaxon:7215
)
Strain
Canton S
esg-GAL4, UAS-GFP, tub- Gal80t
hsFLP, UAS-mCD8-GFP; act5C-FRTstopFRT-GAL4, UAS-GFP, UAS-TorTED
Cell
-
Cell line
-
MeSH
Imaginal Discs,
Wings, Animal
(
D000073758
)
Brain
Fat Body
Lysosomes
Ontology
Anatomical Entity
imaginal disc,
wing
(
FBbt:00004729
)
larval brain
(
FBbt:00001920
)
embryonic/larval fat body
(
FBbt:00001704
)
adult gut
(
FBbt:00007513
)
UBERON
Biological Process
-
Cellular Component
lysosome
(
GO:0005764
)
Molecular Function
-
Medical Subject Headings
Imaginal Discs,
Wings, Animal
(
D000073758
)
Brain
Fat Body
Lysosomes
Imaging Method
Method involved in biological imaging
inelastic scattering of photons (Raman scattering)
(
FBbi:00000589
)
stimulated Raman spectroscopy
Paper DOI / Paper URL
People
Contact
Yasuyuki Ozeki
The University of Tokyo
Department of Electrical Engineering and Information Systems
-
-
Imaging dataset contributor
Spencer J Spratt
Takaha Mizuguchi
Hikaru Akaboshi
Hina Kosakamoto
Fumiaki Obata
Quantitative dataset contributor
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Dataset List
Thumbnail
Dataset
Kind
Quantitative
Title
Image data
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The processed Raman images of dMet and background signals in Drosophila larval wing imaginal disc
Image data
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Stimulated Raman scattering images of dMet uptake in Drosophila larval wing imaginal disc
Image data
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The processed Raman images of dMet and background signals in Drosophila larval brain
Image data
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Stimulated Raman scattering images of dMet uptake in Drosophila larval brain
Image data
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The processed Raman images of dMet and background signals in Drosophila larval fat body
Image data
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Stimulated Raman scattering images of dMet uptake in Drosophila larval fat body
Image data
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The processed Raman images of dMet and background signals in Drosophila gut
Image data
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The processed Raman images of dMet and background signals in Drosophila gut
Image data
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Stimulated Raman scattering images of dMet in Drosophila gut after feeding on dMet food for 24 h
Image data
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The processed Raman images of dMet and background signals in Drosophila gut
Image data
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Stimulated Raman scattering images of dMet in Drosophila gut after feeding on dMet food for 48 h
Image data
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The processed Raman images of methyl group and background signals in Drosophila larval fat body
Image data
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The processed Raman images of dMet and background signals in Drosophila larval fat body
Image data
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The processed Raman images of dMet and background signals in wild type Drosophila larval fat body
Image data
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The processed Raman images of dMet and background signals in TorTED-Drosophila larval fat body