SSBD:database v3

320-Spratt-dMetRaman

OMERO gallery,
OMERO index

Project

Title
Stimulated Raman images of deuterated methionine incorporated into adult and larval Drosophila
Description
Introduction: Visualizing small individual biomolecules at subcellular resolution in live cells and tissues can provide valuable insights into metabolic activity in heterogeneous cells, but is challenging. Methods: Here, the authors used stimulated Raman scattering (SRS) microscopy to image deuterated methionine (d-Met) incorporated into Drosophila tissues in vivo. Results: Their results demonstrate that SRS can detect a range of previously uncharacterized cell-to-cell differences in d-Met distribution within a tissue at the subcellular level. Discussion: These results demonstrate the potential of SRS microscopy for metabolic imaging of less abundant but important amino acids such as methionine in tissue.
License
CC BY
Released at
Oct. 20, 2025, midnight
Updated at
Funding
This work was supported in part by Japan Society for the Promotion of Science KAKENHI under JP20H02650, JP20H05725, JP20H05726, and JP21J00452; in part by Japan Science and Technology Agency Core Research for Evolutional Science and Technology (CREST) under Grant JPMJCR 1872; in part by Nakatani Foundation Grant for Technology Development Research, and in part by Quantum Leap Flagship Program of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) under Grant JPMXS0118067246.
# of image datasets
20

Papers

Papers
Paper information: Spratt SJ, Mizuguchi T, Akaboshi H, Kosakamoto H, Okada R, Obata F, Ozeki Y. Imaging the uptake of deuterated methionine in Drosophila with stimulated Raman scattering. Front Chem. 2023 Mar 29;11:1141920.,
Pubmed ID: 37065821,
PMCID: PMC10090404,
DOI: https://doi.org/10.3389/fchem.2023.1141920,
URL: https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2023.1141920/full

Contacts

Contacts
Yasuyuki Ozeki (Contact) , Department of Electrical Engineering and Information Systems , The University of Tokyo
Spencer J Spratt (Imaging Contributor)
Takaha Mizuguchi (Imaging Contributor)
Hikaru Akaboshi (Imaging Contributor)
Hina Kosakamoto (Imaging Contributor)
Fumiaki Obata (Imaging Contributor)

Biosamples

Organisms
Drosophila (NCBI:txid7215)
Strains
esg-GAL4, UAS-GFP, tub- Gal80t
hsFLP, UAS-mCD8-GFP; act5C-FRTstopFRT-GAL4, UAS-GFP, UAS-TorTED
Canton S
Cells

Cell lines

Ontologies

UBERON
gut wall (UBERON_0000328)
wing (UBERON_0000023)
brain (UBERON_0000955)
Anatomical entities
GO: Biological processes
GO: Cellular components
lysosome (GO:0005764)
GO: Molecular functions

Genetics

Gene names
Protein names
Genetic methods
transgenic (EFO_0004020)
Protein tags
Green fluorescent protein from Aequorea (FBbi:00000437)
Probes
probe for lysosomes, LysoTracker (FBbi:00000414)
Oligo Primer
Genotype

Treatments

Reagent or Compound
Concentration
Fold Dilution

Imaging Methods

Dimensions
500x500x1x1x1, 500x500x1x2x1, 500x500x1x3x1,
X scales
80 micrometer,
Y scales
80 micrometer,
Z scales
NA,
T scales
NA,
Channels
2 channel, 1 channel, 3 channel,
Microscopy types
inelastic scattering of photons (Raman scattering) (FBbi:00000589)
Detection methods
Visualization methods
Illumination methods
Sources of contrast
Contrast enhancing methods
Resolution enhancing methods
Imaged parameters
Sample preparation methods

Instruments

Body
Model
Light source
Detector
Objective
Filter set
Dichroic
Body
Home-built
Model
Home-built
Light source
Picosecond Ti:sapphire laser (Coherent, Mira900D) and synchronized Yb fiber laser
Detector
Home-made detector with a Si photodiode (Hamamatsu S3399), electric filters, amplifers, and a lock-in amplifier.
Objective
Olympus 60x, NA1.2
Filter set
Dichroic

Datasets

Dataset name
Organism
Gene / Protein
Dimensions
File size
SSBD:OMERO
Download
Fig2A_Wing_SRSraw
Drosophila (NCBI:txid7215)
500x500x1x1x1
2MB
Gallery, Index
zip
Fig2A_Wing_SRSprocess
Drosophila (NCBI:txid7215)
500x500x1x2x1
90KB
Gallery, Index
zip
Fig2B_Brain_SRSraw
Drosophila (NCBI:txid7215)
500x500x1x1x1
2MB
Gallery, Index
zip
Fig2B_Brain_SRSprocess
Drosophila (NCBI:txid7215)
500x500x1x2x1
88KB
Gallery, Index
zip
Fig2C_FB_SRSraw
Drosophila (NCBI:txid7215)
500x500x1x1x1
2MB
Gallery, Index
zip
Fig2C_FB_SRSprocess
Drosophila (NCBI:txid7215)
500x500x1x2x1
90KB
Gallery, Index
zip
Fig2D_Gut_SRSraw
Drosophila (NCBI:txid7215)
500x500x1x1x1
2MB
Gallery, Index
zip
Fig2D_Gut_SRSprocess
Drosophila (NCBI:txid7215)
500x500x1x2x1
86KB
Gallery, Index
zip
Fig3AB_24h_raw
Drosophila (NCBI:txid7215)
500x500x1x2x1
4MB
Gallery, Index
zip
Fig3AB_24h_process
Drosophila (NCBI:txid7215)
500x500x1x3x1
106KB
Gallery, Index
zip
Fig3CD_48h_raw
Drosophila (NCBI:txid7215)
500x500x1x2x1
4MB
Gallery, Index
zip
Fig3CD_48h_process
Drosophila (NCBI:txid7215)
500x500x1x3x1
115KB
Gallery, Index
zip
Fig4_CH_raw
Drosophila (NCBI:txid7215)
500x500x1x2x1
4MB
Gallery, Index
zip
Fig4_CH_process
Drosophila (NCBI:txid7215)
500x500x1x3x1
94KB
Gallery, Index
zip
Fig4_dMet_raw
Drosophila (NCBI:txid7215)
500x500x1x2x1
4MB
Gallery, Index
zip
Fig4_dMet_process
Drosophila (NCBI:txid7215)
500x500x1x3x1
98KB
Gallery, Index
zip
Fig5A_raw
Drosophila (NCBI:txid7215)
500x500x1x2x1
4MB
Gallery, Index
zip
Fig5A_process
Drosophila (NCBI:txid7215)
500x500x1x3x1
188KB
Gallery, Index
zip
Fig5BC_raw
Drosophila (NCBI:txid7215)
Tor
500x500x1x2x1
4MB
Gallery, Index
zip
Fig5BC_process
Drosophila (NCBI:txid7215)
Tor
500x500x1x3x1
111KB
Gallery, Index
zip