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320-Spratt-dMetRaman/Fig4_dMet_process

Author reviewed
The processed Raman images of dMet and background signals in Drosophila larval fat body
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Project
320-Spratt-dMetRaman
Dataset ID
Fig4_dMet_process
Kind
Image data
Title
The processed Raman images of dMet and background signals in Drosophila larval fat body
Description
The processed Raman images of deuterated methionine (dMet) and background signals in Drosophila larval fat body. XXX_Met.jpg is dMet image, and XXX_XPM.jpg is background image. XXX_Met.jpg was genarated from 1.tif and 2.tif which was subtracted 0.tif and 3.tif background signals. XXX_XPM.jpg was generated from 0.tif and 3.tif background signals. The image of LysoTracker fluorescence in the same area is also reposited.
Folder Name
Fig4_dMet_process
File Format
.jpg
File Size
98KB
File Separation Method
XXX_dMet.jpg is dMet image, XXX_XPM.jpg is background image, and XXX_LysoTracker.jpg is LysoTracker image.
Figure Number
Fig4
Dimensions
500x500x1x3x1
X Scale
80 micrometer
Y Scale
80 micrometer
Z Scale
-
T Scale
-
Channel
3 channel
Quantitative Data
-

Biosample

Description
The dissected fat body from the third instar larvae of Drosophila which was incorporated deuterated methionine (dMet).
Organism
Drosophila (NCBITaxon:7215)
Strain
Canton S
Cell
-
Cell line
-
MeSH
Fat Body (D005216)
Lysosomes (D008247)
Intrinsic Variable
-
Extrinsic Variable
Flies were transferred to a modified version of holidic medium supplemented with deuterated methionine (Cambridge Isotope Laboratories: DLM-6797–0.1) instead of methionine, for up to 48 h prior to dissection.

Ontology

Anatomical Entity
embryonic/larval fat body (FBbt:00001704)
UBERON
-
Biological Process
-
Cellular Component
lysosome (GO:0005764)
Molecular Function
-
MeSH Anatomy
Fat Body (D005216)
Lysosomes (D008247)
MeSH BP
Fat Body (D005216)
Lysosomes (D008247)
MeSH CC
Fat Body (D005216)
Lysosomes (D008247)
MeSH MF
Fat Body (D005216)
Lysosomes (D008247)

Genetics

Gene
-
Protein / Probe
-probe for lysosomes, LysoTracker
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
-
Detection Method
-
Visualization Method
-
Illumination Method
-
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
-
MeSH
Fat Body (D005216)
Lysosomes (D008247)

Instrument

Body
-
Module
-
Light Source
-
Detector
-
Objective
-
Filter Set
-
Dichroic
-