320-Spratt-dMetRaman/Fig3CD_48h_process
Author reviewedThe processed Raman images of dMet and background signals in Drosophila gut
Project
Dataset ID
Fig3CD_48h_process
Kind
Image data
Title
The processed Raman images of dMet and background signals in Drosophila gut
Description
The processed Raman images of deuterated methionine (dMet) and background signals in Drosophila gut. XXX_Met.jpg is dMet image, and XXX_XPM.jpg is background image. XXX_Met.jpg was genarated from 1.tif and 2.tif which was subtracted 0.tif and 3.tif background signals. XXX_XPM.jpg was generated from 0.tif and 3.tif background signals. The images of GFP fluorescence in the same area is also reposited.
Folder Name
Fig3CD_48h_process
File Format
.jpg
File Size
115KB
File Separation Method
XXX_Met.jpg is dMet image, XXX_GFP.jpg is GFP image, and XXX_XPM.jpg is background image.
Figure Number
Fig3CD
Dimensions
500x500x1x3x1
X Scale
80 micrometer
Y Scale
80 micrometer
Z Scale
-
T Scale
-
Channel
3 channel
Quantitative Data
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Biosample
Description
The dissected gut from adult Drosophila which was feeded deuterated methionine (dMet) containing food for 48 hours. GFP was expressed under control of the escargot gene.
Organism
Drosophila (NCBITaxon:7215)
Strain
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Cell
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Cell line
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MeSH
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Intrinsic Variable
GFP was expressed under the control of the escargot gene.
Extrinsic Variable
Flies were transferred to a modified version of holidic medium supplemented with deuterated methionine (Cambridge Isotope Laboratories: DLM-6797–0.1) instead of methionine, for up to 48 h prior to dissection.
Ontology
Anatomical Entity
adult gut (FBbt:00007513)
UBERON
gut wall (UBERON:0000328)
Biological Process
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Cellular Component
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Molecular Function
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MeSH Anatomy
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MeSH BP
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MeSH CC
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MeSH MF
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Genetics
Gene
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Protein / Probe
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Method / Genotype
transgenic / esg-GAL4, UAS-GFP, tub- Gal80t
Treatments
Reagent / Compound
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Concentration
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Fold Dilution
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Imaging Method
Imaging Method
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Detection Method
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Visualization Method
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Illumination Method
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Source of Contrast
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Imaged Parameter
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Sample Preparation Method
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MeSH
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Instrument
Body
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Module
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Light Source
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Detector
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Objective
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Filter Set
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Dichroic
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