289-Fujimoto-MitralCell/FigS6CD_ctrl_pup3_3
Author reviewedTime-lapse calcium imaging of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells without expression of M/T-TeNT in the cells
Project
Dataset ID
FigS6CD_ctrl_pup3_3
Kind
Image data
Title
Time-lapse calcium imaging of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells without expression of M/T-TeNT in the cells
Description
Time-lapse calcium imaging of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells without expression of M/T-TeNT in the cells.
Folder Name
FigS6CD_ctrl_pup3_3
File Format
.oib
File Size
88MB
File Separation Method
-
Figure Number
FigS6CD
Dimensions
256x256x1x1x700
X Scale
1.99 micrometer
Y Scale
1.99 micrometer
Z Scale
-
T Scale
0.429 seconds
Channel
1 channel
Quantitative Data
-
Biosample
Description
Live and anesthetized P6 mouse expressing GCaMP6f in mitral/tufted cells
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
A mouse line Thy1-GCaMP6f (C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J) in which GCaMP6f is expressed in neurons.
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-