289-Fujimoto-MitralCell/FigS6A_ctrl
Author reviewedZ-series images of VAMP2 immunostaining in P6 mouse olfactory bulb
Project
Dataset ID
FigS6A_ctrl
Kind
Image data
Title
Z-series images of VAMP2 immunostaining in P6 mouse olfactory bulb
Description
Z-series images of VAMP2 immunostaining in P6 mouse olfactory bulb.
Folder Name
FigS6A_ctrl
File Format
.oif
File Size
44MB
File Separation Method
-
Figure Number
FigS6A
Dimensions
1024x1024x11x2x1
X Scale
0.621 micrometer
Y Scale
0.621 micrometer
Z Scale
2 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Biosample
Description
A fixed and immunostained olfactory bulb of P6 mouse.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
-
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
-
Cellular Component
cytoplasm (GO:0005737)
presynapse (GO:0098793)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
Vesicle-associated membrane protein 2 / antibody
Method / Genotype
-B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
primary antibody plus labeled secondary antibody
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
cyrostat-sectioned tissue
MeSH
Instrument
Body
Olympus-Evident FV1000
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident UPLSAPO20X
Filter Set
-
Dichroic
-