289-Fujimoto-MitralCell/Fig5ABC_mutDORA-RhoA_NMDA_1
Author reviewedFRET imaging of RhoA activity in mitral cells after NMDA stimulation using the mutDORA-RhoA in the presence of TTX
Project
Dataset ID
Fig5ABC_mutDORA-RhoA_NMDA_1
Kind
Image data
Title
FRET imaging of RhoA activity in mitral cells after NMDA stimulation using the mutDORA-RhoA in the presence of TTX
Description
Time-lapse images of RhoA activity in olfactory bulb slice from P3 mouse. To stimurate NMDA receptors, the slice was treated with NMDA and glycine during imaging. RhoA activity was studied with mutDORA-RhoA, a mutant FRET sensor lacking RhoA binding activity.
Folder Name
Fig5ABC_mutDORA-RhoA_NMDA_1
File Format
.oib
File Size
400MB
File Separation Method
-
Figure Number
Fig5ABC
Dimensions
774x536x1x2x253
X Scale
1.24 micrometer
Y Scale
1.24 micrometer
Z Scale
-
T Scale
5 seconds
Channel
2 channel
Quantitative Data
-
Biosample
Description
Olfactory bulb slice expressing mutant DORA-RhoA. The acute slice excised from P3 mouse brain. The slice was treated with 100 micromolar NMDA and 40 micromolar glycine during imaging.
Organism
Mus musculus (NCBITaxon:10090)
Strain
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
-
Extrinsic Variable
pCAGGS-mutant DORA-RhoA was introduced at E12 by in utero electroporation. The brain slice at P3 was treated with 100 micromolar NMDA and 40 micromolar glycine during imaging.
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
postsynaptic signal transduction (GO:0098926)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
-
Treatments
Reagent / Compound
N-methyl-D-aspartic acid
Concentration
100 micromolar
Fold Dilution
-
Reagent / Compound
glycine
Concentration
40 micromolar
Fold Dilution
-
Reagent / Compound
tetrodotoxin
Concentration
1 micromolar
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
FRET (FBbi:00000367)
Detection Method
-
Visualization Method
mutant DORA-RhoA composed of cpVenus and Cerulean
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XXLPLN25XWMP, NA = 1.05, WD = 2.0
Filter Set
-
Dichroic
-