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289-Fujimoto-MitralCell/Fig5ABC_mutDORA-RhoA_KCl_2 thumbnail

289-Fujimoto-MitralCell/Fig5ABC_mutDORA-RhoA_KCl_2

Author reviewed
FRET imaging of RhoA activity in mitral cells after KCl stimulation using the mutDORA-RhoA in the presence of TTX
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Project
289-Fujimoto-MitralCell
Dataset ID
Fig5ABC_mutDORA-RhoA_KCl_2
Kind
Image data
Title
FRET imaging of RhoA activity in mitral cells after KCl stimulation using the mutDORA-RhoA in the presence of TTX
Description
Time-lapse images of RhoA activity in olfactory bulb slice from P3 mouse, which was stimulated with KCl. RhoA activity was studied with mutDORA-RhoA, a mutant FRET sensor lacking RhoA binding activity.
Folder Name
Fig5ABC_mutDORA-RhoA_KCl_2
File Format
.oib
File Size
498MB
File Separation Method
-
Figure Number
Fig5ABC
Dimensions
1024x504x1x2x253
X Scale
1.24 micrometer
Y Scale
1.24 micrometer
Z Scale
-
T Scale
5 seconds
Channel
2 channel
Quantitative Data
-

Biosample

Description
Olfactory bulb slice expressing mutant DORA-RhoA. The acute slice excised from P3 mouse brain. The slice was treated with 50 mM KCl during imaging.
Organism
Mus musculus (NCBITaxon:10090)
Strain
ICR (https://www.jslc.co.jp/pdf/mouse/002_ICR2013.pdf)
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
rhoA GTP-Binding Protein (D020742)
Cell Body (D066146)
Fluorescence Resonance Energy Transfer (D031541)
Brain (D001921)
Dendrites (D003712)
Intrinsic Variable
-
Extrinsic Variable
pCAGGS-mutant DORA-RhoA was introduced at E12 by in utero electroporation. The slice was treated with 50 mM KCl during imaging.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
postsynaptic signal transduction (GO:0098926)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
rhoA GTP-Binding Protein (D020742)
Cell Body (D066146)
Fluorescence Resonance Energy Transfer (D031541)
Brain (D001921)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
rhoA GTP-Binding Protein (D020742)
Cell Body (D066146)
Fluorescence Resonance Energy Transfer (D031541)
Brain (D001921)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
rhoA GTP-Binding Protein (D020742)
Cell Body (D066146)
Fluorescence Resonance Energy Transfer (D031541)
Brain (D001921)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
rhoA GTP-Binding Protein (D020742)
Cell Body (D066146)
Fluorescence Resonance Energy Transfer (D031541)
Brain (D001921)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
-

Treatments

Reagent / Compound
potassium chloride
Concentration
50 millimolar
Fold Dilution
-
Reagent / Compound
tetrodotoxin
Concentration
1 micromolar
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
FRET (FBbi:00000367)
Detection Method
-
Visualization Method
mutant DORA-RhoA composed of cpVenus and Cerulean
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
rhoA GTP-Binding Protein (D020742)
Cell Body (D066146)
Fluorescence Resonance Energy Transfer (D031541)
Brain (D001921)
Dendrites (D003712)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XXLPLN25XWMP, NA = 1.05, WD = 2.0
Filter Set
-
Dichroic
-