289-Fujimoto-MitralCell/Fig5ABC_DORA-RhoA_NMDAap5_6
Author reviewedFRET imaging of RhoA activity in mitral cells after NMDA stimulation using the DORA-RhoA biosensor in the presence of TTX and AP5
Project
Dataset ID
Fig5ABC_DORA-RhoA_NMDAap5_6
Kind
Image data
Title
FRET imaging of RhoA activity in mitral cells after NMDA stimulation using the DORA-RhoA biosensor in the presence of TTX and AP5
Description
Time-lapse images of RhoA activity in olfactory bulb slice from P3 mouse. To stimurate and block NMDA receptors, the slice was treated with NMDA, glycine, and AP5 during imaging. RhoA activity was studied with DORA-RhoA, a FRET biosensor for RhoA.
Folder Name
Fig5ABC_DORA-RhoA_NMDAap5_6
File Format
.oib
File Size
221MB
File Separation Method
-
Figure Number
Fig5ABC
Dimensions
710x564x1x2x253
X Scale
0.497 micrometer
Y Scale
0.497 micrometer
Z Scale
-
T Scale
5 seconds
Channel
2 channel
Quantitative Data
-
Biosample
Description
Olfactory bulb slice expressing DORA-RhoA. The acute slice excised from P3 mouse brain. The slice was treated with 100 micromolar NMDA, 40 micromolar glycine, and 100 micromolar AP5 during imaging.
Organism
Mus musculus (NCBITaxon:10090)
Strain
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
-
Extrinsic Variable
pCAGGS-DORA-RhoA was introduced at E12 by in utero electroporation. The slice was treated with 100 micromolar NMDA, 40 micromolar glycine, and 100 micromolar AP5 during imaging.
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
postsynaptic signal transduction (GO:0098926)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
-
Treatments
Reagent / Compound
N-methyl-D-aspartic acid
Concentration
100 micromolar
Fold Dilution
-
Reagent / Compound
glycine
Concentration
40 micromolar
Fold Dilution
-
Reagent / Compound
2-amino-5-phosphonopentanoic acid
Concentration
100 micromolar
Fold Dilution
-
Reagent / Compound
tetrodotoxin
Concentration
1 micromolar
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
FRET (FBbi:00000367)
Detection Method
-
Visualization Method
DORA-RhoA composed of cpVenus and Cerulean
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XXLPLN25XWMP, NA = 1.05, WD = 2.0
Filter Set
-
Dichroic
-