289-Fujimoto-MitralCell/Fig4H_ctrl_1
Author reviewedZ-series images of P6 mouse brain in which mitral cells were labed by tdTomato
Project
Dataset ID
Fig4H_ctrl_1
Kind
Image data
Title
Z-series images of P6 mouse brain in which mitral cells were labed by tdTomato
Description
Z-series images of P6 mouse brain in which mitral cells were labed by tdTomato. The control experiment for triple knockout of RhoA, RhoB, and RhoC. The cleared olfactory bulb was stained with DAPI.
Folder Name
Fig4H_ctrl_1
File Format
.lif
File Size
684MB
File Separation Method
-
Figure Number
Fig4H
Dimensions
3070x1024x114x2x1
X Scale
0.568 micrometer
Y Scale
0.569 micrometer
Z Scale
5 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Biosample
Description
A fixed and cleared olfactory bulb of P6 mouse. tdTomato was expressed in mitral/tufted cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
-
Extrinsic Variable
By using in utero electroporation, the authors electroporated a pCAFNF-tdTomato and pCAG-FLPo at E12.
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transfection
electroporation
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
tdTomatoFP
4',6-diamidino-2-phenylindole (DAPI)
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
vibratome-sectioned tissue
tissue clearing
MeSH
Instrument
Body
Leica DMi8
Module
-
Light Source
-
Detector
-
Objective
Leica HC PL APO 20x/0,75 IMM CORR CS2
Filter Set
-
Dichroic
-