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289-Fujimoto-MitralCell/Fig1G_P3_ctrl thumbnail

289-Fujimoto-MitralCell/Fig1G_P3_ctrl

Author reviewed
Z-series images of P3 mouse olfactory bulb without overexpression of Kir2.1
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Project
289-Fujimoto-MitralCell
Dataset ID
Fig1G_P3_ctrl
Kind
Image data
Title
Z-series images of P3 mouse olfactory bulb without overexpression of Kir2.1
Description
Z-series images of P3 mouse brain. By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato) and a small amount of Cre plasmid (pCAG-iCre) at E12 to label a subset of mitral cells sparsely. At P3 the brain was sliced and cleared with SeeDB2, and staind with DAPI.
Folder Name
Fig1G_P3_ctrl
File Format
.oib
File Size
26MB
File Separation Method
-
Figure Number
Fig1G
Dimensions
598x598x19x2x1
X Scale
0.621 micrometer
Y Scale
0.621 micrometer
Z Scale
5 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-

Biosample

Description
A fixed and cleared olfactory bulb of P3 mouse. tdTomato was expressed in mitral cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
ICR (https://www.jslc.co.jp/pdf/mouse/002_ICR2013.pdf)
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
Intrinsic Variable
-
Extrinsic Variable
By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato) and Cre plasmid (pCAG-Cre) at E12.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation
Gene
-
Protein / Probe
-4',6-diamidino-2-phenylindole (DAPI)
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
tdTomatoFP 4',6-diamidino-2-phenylindole (DAPI)
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue vibratome-sectioned tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Instrument

Body
Olympus-Evident FV1000
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident UPLSAPO20X
Filter Set
-
Dichroic
-