191-Shima-KIF5Cmove/FigS3AB_MTcompact
Author reviewedTime-series images of GDP-MT and KIF5C before and after washout of KIF5C
Project
Dataset ID
FigS3AB_MTcompact
Kind
Image data
Title
Time-series images of GDP-MT and KIF5C before and after washout of KIF5C
Description
Time-series images of GDP-MT (GDP-microtubules) and monomeric KIF5C (K351) before and after washout of KIF5C, which were obtained by fluorescent speckle microscopy.
Folder Name
FigS3AB_MTcompact
File Format
tif
File Size
45MB
File Separation Method
before and after washout of KIF5C
Figure Number
FigS3AB
Dimensions
512x512x1x2x71
X Scale
60 nanometer/pixel
Y Scale
60 nanometer/pixel
Z Scale
-
T Scale
2 seconds per time interval,
5 seconds per time interval
Channel
2 channel
Quantitative Data
-
Biosample
Description
Immobilized microtubule seeds, which were labeled with tetramethylrhodamine, on a glass, and KIF5C were labeled with DY-647
Organism
-
Strain
-
Cell
-
Cell line
-
MeSH
Time-Lapse Imaging (D059008)
Intrinsic Variable
-
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
microtubule depolymerization (GO:0007019)
Cellular Component
-
Molecular Function
-
MeSH Anatomy
Time-Lapse Imaging (D059008)
MeSH BP
Time-Lapse Imaging (D059008)
MeSH CC
Time-Lapse Imaging (D059008)
MeSH MF
Time-Lapse Imaging (D059008)
Genetics
Gene
-
Protein / Probe
Tubulin alpha-1A chain, Tubulin beta chain / Tetramethylrhodamine succinimidyl ester
Method / Genotype
purified from porcine brain through four cycles of polymerization and depolymerization
Gene
-
Protein / Probe
Kinesin heavy chain isoform 5C / DY-647-maleimide
Method / Genotype
in vitro translation and purification
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
evanescent wave microscopy (FBbi:00000617)
time lapse microscopy (FBbi:00000249)
Detection Method
electron multiplying CCD (EMCCD)
Visualization Method
Tetramethylrhodamine (probe for microtubules) and DY-647 (probe for KIF5C)
Illumination Method
continuous illumination
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
-
MeSH
Time-Lapse Imaging (D059008)
Instrument
Body
Olympus-Evident IX83
Module
-
Light Source
Coherent, Obis LX 532 (for microtubule) and Cooherent, CUBE 640 (for KIF5C)
Detector
iXon3 EM CCD camera
Objective
UP- lanSApo, NA 1.40, oil; Olympus
Filter Set
-
Dichroic
-