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191-Shima-KIF5Cmove

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Title

KIF5C running on various states of microtubules

Description

Kinesin-1, the founding member of the kinesin superfamily of proteins, is known to use only a subset of microtubules for transport in living cells. This biased use of microtubules is proposed as the guidance cue for polarized transport in neurons, but the underlying mechanisms are still poorly understood. Here, we report that kinesin-1 binding changes the microtubule lattice and promotes further kinesin-1 binding. This high-affinity state requires the binding of kinesin-1 in the nucleotide- free state. Microtubules return to the initial low-affinity state by washing out the binding kinesin-1 or by the binding of non-hydrolyzable ATP analogue AMPPNP to kinesin-1. X-ray fiber diffraction, fluorescence speckle microscopy, and second- harmonic generation microscopy, as well as cryo-EM, collectively demonstrated that the binding of nucleotide-free kinesin-1 to GDP microtubules changes the conformation of the GDP microtubule to a conformation resembling the GTP microtubule.

License

CC BY

Released at

Oct. 20, 2025, midnight

Updated at

Funding

This work was supported by the Ministry of Education, Cul- ture, Sports, Science and Technology through a Grant-in-Aid for Specially Promoted Research (grant 23000013 to N. Hi- rokawa) and Grant-in-Aid for Scientific Research (KAKENHI grant 16H06372 to N. Hirokawa; grants 16H05119, 15H01334, 26115721, 26650069, and 25293046 to Y. Okada; grants 15H01656 and 17H05897 to H. Shigematsu; and grant 15K08168 to R. Nitta), the Uehara Memorial Foundation (Y. Okada), the Takeda Science Foundation (Y. Okada and R. Nitta), the Mochida Memorial Foun- dation for Medical and Pharmaceutical Research (R. Nitta), the RIKEN Special Postdoctoral Researchers Program (T. Shima), the All RIKEN Research Project on Single Cell (Y. Okada), and the RIKEN Pioneering Project on Dynamic Structural Biology (Y. Okada, H. Shigematsu, and M. Shirouzu).

# of image datasets

6

Papers

Papers

Paper information: Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N. Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport. J Cell Biol. 2018 Dec 3;217(12):4164-4183.,
Pubmed ID: 30297389,
PMCID: PMC6279379,
DOI: https://doi.org/10.1083/jcb.201711178,
URL: https://pubmed.ncbi.nlm.nih.gov/30297389/

Contacts

Contacts

Yasushi Okada (Imaging Contributor) , Laboratory for Cell Polarity Regulation , RIKEN BDR
Nobutaka Hirokawa (Contact) , Department of Cell Biology and Anatomy, Graduate School of Medicine , The University of Tokyo
Tomohiro Shima (Imaging Contributor)
Sotaro Uemura (Imaging Contributor)

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