191-Shima-KIF5Cmove
Author reviewedKIF5C running on various states of microtubules
Project ID
191-Shima-KIF5Cmove
Project SID
191
Title
KIF5C running on various states of microtubules
Description
Kinesin-1, the founding member of the kinesin superfamily of proteins, is known to use only a subset of microtubules for transport in living cells. This biased use of microtubules is proposed as the guidance cue for polarized transport in neurons, but the underlying mechanisms are still poorly understood. Here, we report that kinesin-1 binding changes the microtubule lattice and promotes further kinesin-1 binding. This high-affinity state requires the binding of kinesin-1 in the nucleotide- free state. Microtubules return to the initial low-affinity state by washing out the binding kinesin-1 or by the binding of non-hydrolyzable ATP analogue AMPPNP to kinesin-1. X-ray fiber diffraction, fluorescence speckle microscopy, and second- harmonic generation microscopy, as well as cryo-EM, collectively demonstrated that the binding of nucleotide-free kinesin-1 to GDP microtubules changes the conformation of the GDP microtubule to a conformation resembling the GTP microtubule.
Submission Date
2023-12-18
Opened Date
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Release Date
2024-12-14
Update Date
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Kind
Image datasets
License
Project URL
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Project DOI
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Metadata Version
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Template Version
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Funding Information
This work was supported by the Ministry of Education, Cul- ture, Sports, Science and Technology through a Grant-in-Aid for Specially Promoted Research (grant 23000013 to N. Hi- rokawa) and Grant-in-Aid for Scientific Research (KAKENHI grant 16H06372 to N. Hirokawa; grants 16H05119, 15H01334, 26115721, 26650069, and 25293046 to Y. Okada; grants 15H01656 and 17H05897 to H. Shigematsu; and grant 15K08168 to R. Nitta), the Uehara Memorial Foundation (Y. Okada), the Takeda Science Foundation (Y. Okada and R. Nitta), the Mochida Memorial Foun- dation for Medical and Pharmaceutical Research (R. Nitta), the RIKEN Special Postdoctoral Researchers Program (T. Shima), the All RIKEN Research Project on Single Cell (Y. Okada), and the RIKEN Pioneering Project on Dynamic Structural Biology (Y. Okada, H. Shigematsu, and M. Shirouzu).
Biosample
Organism
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Strain
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Cell
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Cell line
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MeSH
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D059008
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Biological Transport, Active,
Ontology
Anatomical Entity
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UBERON
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Biological Process
microtubule-based transport
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GO:0099111
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microtubule bundle maintenance
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GO:0062195
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Cellular Component
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Molecular Function
microtubule motor activity
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GO:0003777
)
Medical Subject Headings
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D059008
)
Biological Transport, Active,
Imaging Method
Method involved in biological imaging
evanescent wave microscopy
(
FBbi:00000617
)
time lapse microscopy
(
FBbi:00000249
)
Paper DOI / Paper URL
People
Contact
Yasushi Okada
RIKEN BDR
Laboratory for Cell Polarity Regulation
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Nobutaka Hirokawa
The University of Tokyo
Department of Cell Biology and Anatomy, Graduate School of Medicine
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Imaging dataset contributor
Tomohiro Shima
Sotaro Uemura
Yasushi Okada
Quantitative dataset contributor
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Dataset List
Thumbnail
Dataset
Kind
Quantitative
Title
Image data
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Time-series images of x-ray fiber diffraction measuring axial pitch of GDP-MT before and after addition of K11C or AMPPMP
Image data
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Time-series images of x-ray fiber diffraction measuring axial pitch of GDP-MT before and after addition of K11C or AMPPMP
Image data
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Time-series images of x-ray fiber diffraction measuring axial pitch of GDP-MT before and after addition of K11C or AMPPMP
Image data
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Time-series images of GDP-MT and KIF5C before and after washout of KIF5C