Description
To monitor syncytium formation, two lines of ESC that were marked with different fluorescent proteins (H2B-Venus and mCherry, respectively) were independently treated with AP for 4 days, and then mixed and cocultured. After 4–6 days of coculture, the appearance of syncytia, defined as cells expressing both the Venus signals in the nucleus and mCherry signals in the cell body, was detected.
No sibling records.