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180-Watabe-Biosensoractivity
Author reviewed
Imges of different biosensors activity expressed in HeLa cells, MDCK cells or mice ear skin.
SSBD:OMERO.gallery
SSBD:OMERO
SSBD:downloads
Project ID
180-Watabe-Biosensoractivity
Title
Imges of different biosensors activity expressed in HeLa cells, MDCK cells or mice ear skin.
Description
-
Release Date
2022-03-31
Update Date
-
License
CC BY
Version
1.0.0
Method Summary
See details in Watabe T, et. al. (2020) ACS Sens., 5(3):719-730.
Paper DOI
https://doi.org/10.1021/acssensors.9b01941
Paper URL
https://www.ncbi.nlm.nih.gov/pubmed/32101394
Kind
Image data
Total
3998 files / 6.6 GB
Image datasets
zipped 115 files / 2.8 GB, raw image 3883 files / 3.9 GB, total 3998 files / 6.6 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Organism
Homo sapiens (NCBI:txid9606 )
Strain
-
Cell line
HeLa cell (
CLO:0003684
)
Biological Process
-
Cellular Component
-
Medical Subject Headings
Adenylyl Cyclases (
MeSH:D000262
)
Animals (
MeSH:D000818
)
Bacterial Proteins (
MeSH:D001426
)
Biosensing Techniques (
MeSH:D015374
)
Cyclic AMP-Dependent Protein Kinases (
MeSH:D017868
)
Cyclic AMP-Dependent Protein Kinases/genetics (
MeSH:Q000235
)
Cyclic AMP-Dependent Protein Kinases/metabolism (
MeSH:Q000378
)
Dogs (
MeSH:D004285
)
Female (
MeSH:D005260
)
Fluorescence Resonance Energy Transfer (
MeSH:D031541
)
Green Fluorescent Proteins (
MeSH:D049452
)
HEK293 Cells (
MeSH:D057809
)
HeLa Cells (
MeSH:D006367
)
Humans (
MeSH:D006801
)
Light (
MeSH:D008027
)
Luminescent Proteins (
MeSH:D008164
)
Madin Darby Canine Kidney Cells (
MeSH:D061985
)
Male (
MeSH:D008297
)
Mice
Transgenic (
MeSH:D008822
)
Optogenetics (
MeSH:D062308
)
Spectrometry
Fluorescence (
MeSH:D013050
)
Method involved in biological imaging
differences in fluorescence lifetime (
FBbi:00000605
)
FRET (
FBbi:00000367
)
time lapse microscopy (
FBbi:00000249
)
Image datasets
115
Quantitative datasets
0
Paper DOI / Paper URL
PMID: 32101394
,
doi: 10.1021/acssensors.9b01941
OMERO.gallery
https://ssbd.riken.jp/omero/gallery/show_project/706/
OMERO
https://ssbd.riken.jp/omero/webclient/?show=project-706
downloads
https://ssbd.riken.jp/data/180-Watabe-Biosensoractivity/
People
Contact
Kenta Terai
Graduate School of Medicine, Kyoto University
Department of Pathology and Biology of Diseases
-
Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8315 Japan
Imaging dataset contributor
-
Quantitative dataset contributor
-
Datasets
Previous
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Thumbnail
Dataset
Organism
Kind / Links
Fig1B_No10_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No10_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No11_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No11_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No12_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No12_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No13_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No13_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No14_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No14_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No15_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No15_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No16_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No16_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No1_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No1_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No2_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No2_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No3_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No3_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No4_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No4_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No5_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No5_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No6_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No6_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No7_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No7_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No8_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No8_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig1B_No9_afterFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Fig1B_No9_beforeFSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Fig2AB_HeLa_3559NES_AKAR3EV
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Time-lapse images of HeLa cells expressing AKAR3EV stimulated with 50 μM forskolin (FSK).
Fig2AB_HeLa_4493NES_BoosterPKA
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Time-lapse images of HeLa cells expressing Booster- PKA stimulated with 50 μM forskolin (FSK).
Fig2AB_HeLa_4589NES_PKA_TAmutant
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Time-lapse images of HeLa cells expressing the phosphorylation-deficient TA mutant Booster-PKA (TA) stimulated with 50 μM forskolin (FSK)..
Figure3A_Booster_JNK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Time-lapse images of HeLa cells expressing the Booster-JNK before and 40 min after 1 μg/mL anisomycin stimulation.
Figure3B_Booster_ROCK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Time-lapse images of HeLa cells expressing the Booster-ROCK 2 min after 10 ng/mL EGF stimulation and 10 min after 30 μM Y27632 treatment.
Figure3C_Booster_ERK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Time-lapse images of HeLa cells expressing the Booster-ERK before stimulation and 10 min after 10 ng/mL EGF stimulation.
Figure4C_HeLa_EKAREV_Booster-PKA
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Time-lapse images of HeLa cells expressing both EKAREV and Booster-PKA.
Figure5A_FSK_HeLa_FSKtiteration1_thumb_w1CFP
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Figure5A_FSK_HeLa_FSKtiteration1_thumb_w2FRET
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Figure5A_FSK_HeLa_FSKtiteration1_thumb_w3mKO2
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Figure5A_FSK_HeLa_FSKtiteration1_thumb_w4mKate2-FRET
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Figure5A_FSK_HeLa_FSKtiteration1_thumb_w5DIC-fast
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Figure5A_FSK_HeLa_FSKtiteration1_w1CFP
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Figure5A_FSK_HeLa_FSKtiteration1_w2FRET
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Figure5A_FSK_HeLa_FSKtiteration1_w3mKO2
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Figure5A_FSK_HeLa_FSKtiteration1_w4mKate2-FRET
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Figure5A_FSK_HeLa_FSKtiteration1_w5DIC-fast
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Figure5B_ISO_HeLa_Isotiteration_thumb_w1CFP
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of isoproterenol (ISO)