180-Watabe-Biosensoractivity thumbnail

180-Watabe-Biosensoractivity

Author reviewed
Imges of different biosensors activity expressed in HeLa cells, MDCK cells or mice ear skin.
Project ID
180-Watabe-Biosensoractivity
Title
Imges of different biosensors activity expressed in HeLa cells, MDCK cells or mice ear skin.
Description
-
Release Date
2022-03-31
Update Date
-
License
Version
1.0.0
Method Summary
See details in Watabe T, et. al. (2020) ACS Sens., 5(3):719-730.
Paper DOI
Paper URL
Kind
Image data
Total
3998 files / 6.6 GB
Image datasets
zipped 115 files / 2.8 GB, raw image 3883 files / 3.9 GB, total 3998 files / 6.6 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Organism
Homo sapiens (NCBI:txid9606 )
Strain
-
Cell line
HeLa cell ( CLO:0003684 )
Biological Process
-
Cellular Component
-
Medical Subject Headings
Adenylyl Cyclases ( MeSH:D000262 )
Animals ( MeSH:D000818 )
Bacterial Proteins ( MeSH:D001426 )
Biosensing Techniques ( MeSH:D015374 )
Cyclic AMP-Dependent Protein Kinases ( MeSH:D017868 )
Cyclic AMP-Dependent Protein Kinases/genetics ( MeSH:Q000235 )
Cyclic AMP-Dependent Protein Kinases/metabolism ( MeSH:Q000378 )
Dogs ( MeSH:D004285 )
Female ( MeSH:D005260 )
Fluorescence Resonance Energy Transfer ( MeSH:D031541 )
Green Fluorescent Proteins ( MeSH:D049452 )
HEK293 Cells ( MeSH:D057809 )
HeLa Cells ( MeSH:D006367 )
Humans ( MeSH:D006801 )
Light ( MeSH:D008027 )
Luminescent Proteins ( MeSH:D008164 )
Madin Darby Canine Kidney Cells ( MeSH:D061985 )
Male ( MeSH:D008297 )
Mice
Transgenic ( MeSH:D008822 )
Optogenetics ( MeSH:D062308 )
Spectrometry
Fluorescence ( MeSH:D013050 )
Method involved in biological imaging
differences in fluorescence lifetime ( FBbi:00000605 )
FRET ( FBbi:00000367 )
time lapse microscopy ( FBbi:00000249 )

Image datasets
115
Quantitative datasets
0

Paper DOI / Paper URL

OMERO.gallery
OMERO
downloads

People
Contact
Kenta Terai
Graduate School of Medicine, Kyoto University
Department of Pathology and Biology of Diseases
-
Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8315 Japan
Imaging dataset contributor
-
Quantitative dataset contributor
-

Datasets

Thumbnail
Dataset
Organism
Kind / Links
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells 10 min after 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Lifetime images of Booster-PKA expressed in HeLa cells before 50 μM FSK stimulation.
Homo sapiens (NCBI:txid9606 )
Time-lapse images of HeLa cells expressing AKAR3EV stimulated with 50 μM forskolin (FSK).
Homo sapiens (NCBI:txid9606 )
Time-lapse images of HeLa cells expressing Booster- PKA stimulated with 50 μM forskolin (FSK).
Homo sapiens (NCBI:txid9606 )
Time-lapse images of HeLa cells expressing the phosphorylation-deficient TA mutant Booster-PKA (TA) stimulated with 50 μM forskolin (FSK)..
Homo sapiens (NCBI:txid9606 )
Time-lapse images of HeLa cells expressing the Booster-JNK before and 40 min after 1 μg/mL anisomycin stimulation.
Homo sapiens (NCBI:txid9606 )
Time-lapse images of HeLa cells expressing the Booster-ROCK 2 min after 10 ng/mL EGF stimulation and 10 min after 30 μM Y27632 treatment.
Homo sapiens (NCBI:txid9606 )
Time-lapse images of HeLa cells expressing the Booster-ERK before stimulation and 10 min after 10 ng/mL EGF stimulation.
Homo sapiens (NCBI:txid9606 )
Time-lapse images of HeLa cells expressing both EKAREV and Booster-PKA.
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Homo sapiens (NCBI:txid9606 )
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Homo sapiens (NCBI:txid9606 )
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Homo sapiens (NCBI:txid9606 )
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of forskolin (FSK)
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of isoproterenol (ISO)