SSBD
:database
Share your data
SSBD:repository
SSBD:database
Resources
Resources in SSBD:database (metadata v3)
Resources in SSBD:database
Resources in SSBD:repository
SSBD:OMERO
SSBD:BFF
Tools
Ontology validator
Publications
About
About SSBD
Statistics
About us
News
Copyright
Usage
180-Watabe-Biosensoractivity
Author reviewed
Imges of different biosensors activity expressed in HeLa cells, MDCK cells or mice ear skin.
SSBD:OMERO.gallery
SSBD:OMERO
SSBD:downloads
Project ID
180-Watabe-Biosensoractivity
Title
Imges of different biosensors activity expressed in HeLa cells, MDCK cells or mice ear skin.
Description
-
Release Date
2022-03-31
Update Date
-
License
CC BY
Version
1.0.0
Method Summary
See details in Watabe T, et. al. (2020) ACS Sens., 5(3):719-730.
Paper DOI
https://doi.org/10.1021/acssensors.9b01941
Paper URL
https://www.ncbi.nlm.nih.gov/pubmed/32101394
Kind
Image data
Total
3998 files / 6.6 GB
Image datasets
zipped 115 files / 2.8 GB, raw image 3883 files / 3.9 GB, total 3998 files / 6.6 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Organism
Homo sapiens (NCBI:txid9606 )
Canis lupus familiaris
(
NCBITaxon:9615
)
Mus musculus
(
NCBITaxon:10090
)
Strain
-
Cell line
HeLa cell ( CLO:0003684 )
MDCK cell ( CLO:0007646 )
Biological Process
-
Cellular Component
-
Medical Subject Headings
-
Method involved in biological imaging
FRET (
FBbi:00000367
)
fluorescence microscopy (
FBbi:00000246
)
differences in fluorescence lifetime (
FBbi:00000605
)
Image datasets
115
Quantitative datasets
0
Paper DOI / Paper URL
PMID: 32101394
,
doi: 10.1021/acssensors.9b01941
OMERO.gallery
https://ssbd.riken.jp/omero/gallery/show_project/706/
OMERO
https://ssbd.riken.jp/omero/webclient/?show=project-706
downloads
https://ssbd.riken.jp/data/180-Watabe-Biosensoractivity/
People
Contact
Kenta Terai
Graduate School of Medicine, Kyoto University
Department of Pathology and Biology of Diseases
-
Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8315 Japan
Imaging dataset contributor
-
Quantitative dataset contributor
-
Datasets
Previous
1
2
3
Next
Thumbnail
Dataset
Organism
Kind / Links
Figure5B_ISO_HeLa_Isotiteration_thumb_w2FRET
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of isoproterenol (ISO)
Figure5B_ISO_HeLa_Isotiteration_thumb_w3mKO2
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of isoproterenol (ISO)
Figure5B_ISO_HeLa_Isotiteration_thumb_w4mKate2-FRET
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of isoproterenol (ISO)
Figure5B_ISO_HeLa_Isotiteration_thumb_w5DIC-fast
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of isoproterenol (ISO)
Figure5B_ISO_HeLa_Isotiteration_w1CFP
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of isoproterenol (ISO)
Figure5B_ISO_HeLa_Isotiteration_w2FRET
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of isoproterenol (ISO)
Figure5B_ISO_HeLa_Isotiteration_w3mKO2
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of isoproterenol (ISO)
Figure5B_ISO_HeLa_Isotiteration_w4mKate2-FRET
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of isoproterenol (ISO)
Figure5B_ISO_HeLa_Isotiteration_w5DIC-fast
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both hyBRET-Epac and Booster-PKA stimulated with increasing amounts of isoproterenol (ISO)
Figure6B_bPAC_44934589_thumb_w2mKO2_s1
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both Booster- PKA and bPAC.
Figure6B_bPAC_44934589_thumb_w2mKO2_s2
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both Booster- PKA and bPAC.
Figure6B_bPAC_44934589_thumb_w2mKO2_s3
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both Booster- PKA and bPAC.
Figure6B_bPAC_44934589_thumb_w3mKate2-FRET_s1
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both Booster- PKA and bPAC.
Figure6B_bPAC_44934589_thumb_w3mKate2-FRET_s2
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both Booster- PKA and bPAC.
Figure6B_bPAC_44934589_thumb_w3mKate2-FRET_s3
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both Booster- PKA and bPAC.
Figure6B_bPAC_44934589_w2mKO2_s1
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both Booster- PKA and bPAC.
Figure6B_bPAC_44934589_w2mKO2_s2
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both Booster- PKA and bPAC.
Figure6B_bPAC_44934589_w2mKO2_s3
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both Booster- PKA and bPAC.
Figure6B_bPAC_44934589_w3mKate2-FRET_s1
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both Booster- PKA and bPAC.
Figure6B_bPAC_44934589_w3mKate2-FRET_s2
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both Booster- PKA and bPAC.
Figure6B_bPAC_44934589_w3mKate2-FRET_s3
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Images of HeLa cells expressing both Booster- PKA and bPAC.
Figure6C_mKOk_raw_311-326
Canis lupus familiaris
(
NCBITaxon:9615
)
Image data
omero.gallery
omero
Images of MDCK cells expressing Booster-PKA were cocultured with MDCK cells expressing bPAC-P2A-mCherry- NLS.
Figure6C_mKate2FRET_raw_311-326
Canis lupus familiaris
(
NCBITaxon:9615
)
Image data
omero.gallery
omero
Images of MDCK cells expressing Booster-PKA were cocultured with MDCK cells expressing bPAC-P2A-mCherry- NLS.
Figure7D_EarSkin_dbcAMP
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
In vivo imaging of dbcAMP-induced PKA activation in ear skin cells.
Figure7D_EarSkin_dbcAMP_01
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
In vivo imaging of dbcAMP-induced PKA activation in ear skin cells.
Figure7D_EarSkin_dbcAMP_02
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
In vivo imaging of dbcAMP-induced PKA activation in ear skin cells.
Figure7D_EarSkin_dbcAMP_03
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
In vivo imaging of dbcAMP-induced PKA activation in ear skin cells.
Figure7E_Crypt_dbcAMP
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Images of dbcAMP-induced PKA activation in the crypt of the ileum.
Figure7E_Crypt_dbcAMP_01
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Images of dbcAMP-induced PKA activation in the crypt of the ileum.
Figure7E_Crypt_dbcAMP_02
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Images of dbcAMP-induced PKA activation in the crypt of the ileum.
FigureS2_HeLa_4514NES
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors without addition of forskolin.
FigureS2_HeLa_4514NES_FSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors before and after addition of forskolin.
FigureS2_HeLa_4515NES
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors without addition of forskolin.
FigureS2_HeLa_4515NES_FSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors before and after addition of forskolin.
FigureS2_HeLa_4516NES
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors without addition of forskolin.
FigureS2_HeLa_4516NES_FSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors before and after addition of forskolin.
FigureS2_HeLa_4517NES
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors without addition of forskolin.
FigureS2_HeLa_4517NES_FSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors before and after addition of forskolin.
FigureS2_HeLa_4518NES
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors without addition of forskolin.
FigureS2_HeLa_4518NES_FSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors before and after addition of forskolin.
FigureS2_HeLa_4519NES
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors without addition of forskolin.
FigureS2_HeLa_4519NES_FSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors before and after addition of forskolin.
FigureS2_HeLa_4520NES
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors without addition of forskolin.
FigureS2_HeLa_4520NES_FSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors before and after addition of forskolin.
FigureS2_HeLa_4521NES
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors without addition of forskolin.
FigureS2_HeLa_4521NES_FSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors before and after addition of forskolin.
FigureS2_HeLa_4522NES
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors without addition of forskolin.
FigureS2_HeLa_4522NES_FSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors before and after addition of forskolin.
FigureS2_HeLa_4523NES
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors without addition of forskolin.
FigureS2_HeLa_4523NES_FSK
Homo sapiens (NCBI:txid9606 )
Image data
omero.gallery
omero
Fluorescence lifetime images of HeLa cells expressing FRET biosensors before and after addition of forskolin.