129-Komatsu-ERKBiosensorActivity
Author reviewedTimelapse images and BDML file for quantitative information about the extracellular signal-regulated kinase (ERK) biosensor activity in HeLa cells, or 4T1 cancer cells or the transgenic mice
Project ID
129-Komatsu-ERKBiosensorActivity
Title
Timelapse images and BDML file for quantitative information about the extracellular signal-regulated kinase (ERK) biosensor activity in HeLa cells, or 4T1 cancer cells or the transgenic mice
Description
-
Release Date
2021-09-30
Update Date
-
License
Version
1.0.0
Method Summary
See details in Komatsu N, et. al. (2018) Scientific Reports, 8(1): 8984.
Kind
Image data
Total
6569 files / 11.8 GB
Image datasets
zipped 13 files / 5.3 GB, raw image 6556 files / 6.4 GB, total 6569 files / 11.8 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Organism
Mus muculus
(
NCBITaxon:10090
)
Strain
-
Cell line
HeLa cell
(
CLO:0003684
)
4T1 cell
(
CLO:0001401
)
Biological Process
activation of MAPERK kinase
(
GO:0000186
)
Cellular Component
plasma membrane
(
GO:0005886
)
Medical Subject Headings
Animals
(
MeSH:D000818
)
Cell Culture Techniques
(
MeSH:D018929
)
Cell Transformation
Neoplastic
(
MeSH:D002471
)
Neoplastic/genetics
(
MeSH:Q000235
)
Epithelial Cells
(
MeSH:D004847
)
Epithelial Cells/metabolism
(
MeSH:Q000378
)
Extracellular Signal-Regulated MAP Kinases
(
MeSH:D048049
)
Extracellular Signal-Regulated MAP Kinases/genetics
(
MeSH:Q000235
)
Extracellular Signal-Regulated MAP Kinases/metabolism
(
MeSH:Q000378
)
Gene Expression Profiling
(
MeSH:D020869
)
Intestines
(
MeSH:D007422
)
Intestines/cytology
(
MeSH:Q000166
)
Kinetics (MeSH Unique ID:D007700),MAP Kinase Signaling System
(
MeSH:D020935
)
MAP Kinase Signaling System/genetics
(
MeSH:Q000235
)
Mice
Inbred C57BL (MeSH UniqMeSH Unique ID:ue ID:D008810)
Transgenic
(
MeSH:D008822
)
Microscopy
Fluorescence
Multiphoton
(
MeSH:D036641
)
Organoids
(
MeSH:D009940
)
Organoids/cytology
(
MeSH:Q000166
)
Organoids/metabolism
(
MeSH:Q000378
)
Time-Lapse Imaging
(
MeSH:D059008
)
Time-Lapse Imaging/methods
(
MeSH:Q000379
)
Method involved in biological imaging
time lapse microscopy
(
FBbi:00000249
)
Image datasets
13
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Michiyuki Matsuda
Kyoto University
Graduate School of Biostudies
Laboratory of Bioimaging and Cell Signaling
Yoshida-Honmachi, Sakyo-ku, Kyoto 606-8501, Japan
Imaging dataset contributor
-
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse images of HeLa cells expressing FRET-BRET hybrid-biosensor (hyBRET-ERK) stimulated with 10ng/ml EGF
Time-lapse images of HeLa cells expressing FRET-BRET hybrid-biosensor (hyBRET-ERK) and mCherry-CRY2-cRaf
Time-lapse images of HeLa cells expressing FRET-BRET hybrid-biosensor (hyBRET-S6K) and mCherry-Cry2-iSH2
Time-lapse images of the BRET ratio in the 4T1 mammary cancer cells expressing hyBRET-ERK injected Balb/c nude mice administration with MEK inhibitor PD-0325901 (MEKi)
Time-lapse images of BRET ratio in the 4T1 mammary cancer cells expressing hyBRET-ERK injected Balb/c nude mice administration with vehicle alone
Time-lapse FRET images of the TA mutant-expressing lung tumor supplementary with MEK inhibitor PD-0325901 (MEKi)
Time-lapse images of pharmacodynamics of the transgenic mice expressing FRET-BRET hybrid-biosensor (hyBRET-ERK) administration with MEK inhibitor PD-0325901
Time-lapse images of pharmacodynamics of the transgenic mice expressing FRET-BRET hybrid-biosensor (hyBRET-ERK) administration with MEK inhibitor PD-0325901
Time-lapse images of pharmacodynamics of the transgenic mice expressing FRET-BRET hybrid-biosensor (hyBRET-ERK) administration with MEK inhibitor PD-0325901
Time-lapse images of pharmacodynamics of the WT mice administration with MEK inhibitor PD-0325901
Time-lapse images of pharmacodynamics of the WT mice administration with MEK inhibitor PD-0325901
Time-lapse images of pharmacodynamics of the WT mice administration with MEK inhibitor PD-0325901
Time-lapse images of HeLa cells expressing FRET-BRET hybrid-biosensor (hyBRET-ERK) stimulated with 10ng/ml EGF