SSBD:database

129-Komatsu-ERKBiosensorActivity

OMERO gallery,
OMERO index

Project

Title
Timelapse images and BDML file for quantitative information about the extracellular signal-regulated kinase (ERK) biosensor activity in HeLa cells, or 4T1 cancer cells or the transgenic mice
Description
License
CC BY
Released at
Sept. 30, 2021, midnight
Updated at
Funding
# of image datasets
13

Papers

Papers
Paper information: Naoki Komatsu, Kenta Terai, Ayako Imanishi, Yuji Kamioka, Kenta Sumiyama, Takashi Jin, Yasushi Okada, Takeharu Nagai, Michiyuki Matsuda (2018) A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging., Scientific reports, Volume 8, Number 1, pp. 8984,
Pubmed ID: 29895862,
PMCID: 5997707,
DOI: https://doi.org/10.1038/s41598-018-27174-x,

Contacts

Contacts
Michiyuki Matsuda (Contact) , Graduate School of Biostudies , Kyoto University

Biosamples

Organisms
Mus musculus (NCBI:txid10090)
Strains
NA
Cells
Cell lines

HeLa cell (CLO_0003684)
4T1 cell (CLO_0001401)

Anatomical information

UBERON
Anatomical entities

Gene ontology

GO: Biological processes
activation of MAPERK kinase (GO:0000186)
GO: Cellular components
plasma membrane (GO:0005886)
GO: Molecular functions

Molecular biological information

Gene names
Protein names
Genetic methods
Protein tags
Probes
Oligo Primer
Genotype

Reagent or Compound

Reagent or Compound
Concentration
Fold Dilution

Imaging information

Dimensions
X scales
0.662 micrometer/pixel, 0.867 micrometer/pixel, Arbitrary unit by manual variable magnification.,
Y scales
0.662 micrometer/pixel, 0.867 micrometer/pixel, Arbitrary unit by manual variable magnification.,
Z scales
1 micrometer/slice, NA,
T scales
121.91 second for each time interval, 2 minites for each time interval, 1 minite for each time interval, 4 minites for each time interval,
Channels
,

Biological Imaging Methods Ontology

Microscopy types
Detection methods
Visualization methods
Illumination methods
Sources of contrast
Contrast enhancing methods
Resolution enhancing methods
Imaged parameters
Sample preparation methods

Instruments

Datasets

Dataset name
Organism
Gene / Protein
Dimensions
File size
SSBD:OMERO
Download
Figure1C_timelapse_Hela_BRET-ERK
Mus musculus (NCBI:txid10090)
70556051
Gallery, Index
zip
figure1E-H_timelapse
Mus musculus (NCBI:txid10090)
8571216
Gallery, Index
zip
Figure3B_timelapse_Hela_BRET_CRY2Raf
Mus musculus (NCBI:txid10090)
25324578
Gallery, Index
zip
Figure3F_timelapse_BRET
Mus musculus (NCBI:txid10090)
25324576
Gallery, Index
zip
Figure5D_timelapse_BRET_MEKi
Mus musculus (NCBI:txid10090)
37868620
Gallery, Index
zip
Figure5E_timelapse_BRET_vehicle
Mus musculus (NCBI:txid10090)
37868603
Gallery, Index
zip
Figure5H_lungtumor_FRET
Mus musculus (NCBI:txid10090)
87177593
Gallery, Index
zip
Figure5K_MEKi_20170425
Mus musculus (NCBI:txid10090)
1019606585
Gallery, Index
zip
Figure5K_MEKi_20170426
Mus musculus (NCBI:txid10090)
1019607574
Gallery, Index
zip
Figure5K_MEKi_20170512
Mus musculus (NCBI:txid10090)
1273449007
Gallery, Index
zip
Figure5K_WT_20170501
Mus musculus (NCBI:txid10090)
1019632677
Gallery, Index
zip
Figure5K_WT_20170502
Mus musculus (NCBI:txid10090)
1019606460
Gallery, Index
zip
Figure5K_WT_20170510
Mus musculus (NCBI:txid10090)
1273446894
Gallery, Index
zip