129-Komatsu-ERKBiosensorActivity/Figure3F_timelapse_BRET
Author reviewedTime-lapse images of HeLa cells expressing FRET-BRET hybrid-biosensor (hyBRET-S6K) and mCherry-Cry2-iSH2
Dataset ID
Figure3F_timelapse_BRET
Kind
Image data
Title
Time-lapse images of HeLa cells expressing FRET-BRET hybrid-biosensor (hyBRET-S6K) and mCherry-Cry2-iSH2
Description
Time-lapse images of HeLa cells expressing FRET-BRET hybrid-biosensor (hyBRET-S6K) and mCherry-Cry2-iSH2
File or Folder
Figure3F_timelapse_BRET
File Formats
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File Separation Method
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Source
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X-scale
0.867 micrometer/pixel
Y-scale
0.867 micrometer/pixel
Z-scale
-
T-scale
2 minites for each time interval
Organism
Mus muculus
(
NCBITaxon:10090
)
Strain
-
Cell line
HeLa cell
(
CLO:0003684
)
Biological Process
activation of MAPERK kinase
(
GO:0000186
)
Cellular Component
plasma membrane
(
GO:0005886
)
Medical Subject Headings
Animals
(
MeSH:D000818
)
Cell Culture Techniques
(
MeSH:D018929
)
Cell Transformation
Neoplastic
(
MeSH:D002471
)
Cell Transformation
Neoplastic/genetics
(
MeSH:Q000235
)
Epithelial Cells
(
MeSH:D004847
)
Epithelial Cells/metabolism
(
MeSH:Q000378
)
Extracellular Signal-Regulated MAP Kinases
(
MeSH:D048049
)
Extracellular Signal-Regulated MAP Kinases/genetics
(
MeSH:Q000235
)
Extracellular Signal-Regulated MAP Kinases/metabolism
(
MeSH:Q000378
)
Gene Expression Profiling
(
MeSH:D020869
)
Intestines
(
MeSH:D007422
)
Intestines/cytology
(
MeSH:Q000166
)
Kinetics (MeSH Unique ID:D007700),MAP Kinase Signaling System
(
MeSH:D020935
)
MAP Kinase Signaling System/genetics
(
MeSH:Q000235
)
Mice
Inbred C57BL (MeSH UniqMeSH Unique ID:ue ID:D008810)
Mice
Transgenic
(
MeSH:D008822
)
Microscopy
Fluorescence
Multiphoton
(
MeSH:D036641
)
Organoids
(
MeSH:D009940
)
Organoids/cytology
(
MeSH:Q000166
)
Organoids/metabolism
(
MeSH:Q000378
)
Time-Lapse Imaging
(
MeSH:D059008
)
Time-Lapse Imaging/methods
(
MeSH:Q000379
)
Method involved in biological imaging
time lapse microscopy
(
FBbi:00000249
)
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