129-Komatsu-ERKBiosensorActivity
Author reviewedProject name
129-Komatsu-ERKBiosensorActivity
Release date
2021-09-30
Update date
-
Pre-release URL
-
Project title
Timelapse images and BDML file for quantitative information about the extracellular signal-regulated kinase (ERK) biosensor activity in HeLa cells, or 4T1 cancer cells or the transgenic mice
License
Method summary
See details in Komatsu et al. (2018) Scientific Reports, 8(1): 8984.
PubMed URL
Kind
Image data
Organism
Mus muculus
(
NCBITaxon:10090
)
Total
6569 files / 11.8 GB
Image datasets
zipped 13 files / 5.3 GB, raw image 6556 files / 6.4 GB, total 6569 files / 11.8 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Image datasets
13
Quantitative datasets
0
Strain
HeLa
4T1
Datatype
ERK biosensor activity
Basedon
Experiment
XYZ-scale
XY: 0.867 micrometer/pixel
XY: Arbitrary unit by manual variable magnification.
XY: 0.662 micrometer/pixel, Z: 1 micrometer/slice
T-scale
4 minites for each time interval
2 minites for each time interval
1 minite for each time interval
121.91 second for each time interval
dblink
None
External links
None
Experiment type
TimeLapse
Microscope type
FluorescenceMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
IX81, Olympus
Detector model
-
Objective model
UPLAPO 100X/1.35NA oil
Filter set
-
Biological Process
activation of MAPERK kinase
(
GO:0000186
)
Cellular Component
plasma membrane
(
GO:0005886
)
People
Contact
Michiyuki Matsuda
Kyoto University
Graduate School of Biostudies
Laboratory of Bioimaging and Cell Signaling
Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501 Japan
Contributors
Naoki Komatsu, Kenta Terai, Ayako Imanishi, Yuji Kamioka, Kenta Sumiyama, Takashi Jin, Yasushi Okada, Takeharu Nagai, Michiyuki Matsuda
OMERO.gallery
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse images of HeLa cells expressing FRET-BRET hybrid-biosensor (hyBRET-ERK) stimulated with 10ng/ml EGF
Time-lapse images of HeLa cells expressing FRET-BRET hybrid-biosensor (hyBRET-ERK) and mCherry-CRY2-cRaf
Time-lapse images of HeLa cells expressing FRET-BRET hybrid-biosensor (hyBRET-S6K) and mCherry-Cry2-iSH2
Time-lapse images of the BRET ratio in the 4T1 mammary cancer cells expressing hyBRET-ERK injected Balb/c nude mice administration with MEK inhibitor PD-0325901 (MEKi)
Time-lapse images of BRET ratio in the 4T1 mammary cancer cells expressing hyBRET-ERK injected Balb/c nude mice administration with vehicle alone
Time-lapse FRET images of the TA mutant-expressing lung tumor supplementary with MEK inhibitor PD-0325901 (MEKi)
Time-lapse images of pharmacodynamics of the transgenic mice expressing FRET-BRET hybrid-biosensor (hyBRET-ERK) administration with MEK inhibitor PD-0325901
Time-lapse images of pharmacodynamics of the transgenic mice expressing FRET-BRET hybrid-biosensor (hyBRET-ERK) administration with MEK inhibitor PD-0325901
Time-lapse images of pharmacodynamics of the transgenic mice expressing FRET-BRET hybrid-biosensor (hyBRET-ERK) administration with MEK inhibitor PD-0325901
Time-lapse images of pharmacodynamics of the WT mice administration with MEK inhibitor PD-0325901
Time-lapse images of pharmacodynamics of the WT mice administration with MEK inhibitor PD-0325901
Time-lapse images of pharmacodynamics of the WT mice administration with MEK inhibitor PD-0325901
Time-lapse images of HeLa cells expressing FRET-BRET hybrid-biosensor (hyBRET-ERK) stimulated with 10ng/ml EGF