Dataset 129-Komatsu-ERKBiosensorActivity/Figure3F_timelapse_BRET
Author reviewedProject name
Release date
2021-09-30
Update date
-
Pre-release URL
None
Project title
Timelapse images and BDML file for quantitative information about the extracellular signal-regulated kinase (ERK) biosensor activity in HeLa cells, or 4T1 cancer cells or the transgenic mice
Contact name
Michiyuki Matsuda
Organization
Kyoto University
Department
Graduate School of Biostudies
Laboratory
Laboratory of Bioimaging and Cell Signaling
License
Contributors
Naoki Komatsu, Kenta Terai, Ayako Imanishi, Yuji Kamioka, Kenta Sumiyama, Takashi Jin, Yasushi Okada, Takeharu Nagai, Michiyuki Matsuda
Method summary
See details in Komatsu et al. (2018) Scientific Reports, 8(1): 8984.
PubMed URL
File or Folder
Figure3F_timelapse_BRET
Kind
Image data
Title
Time-lapse images of HeLa cells expressing FRET-BRET hybrid-biosensor (hyBRET-S6K) and mCherry-Cry2-iSH2
Description
-
Organism
Mus muculus
(
NCBITaxon:10090
)
Strain
HeLa
Datatype
ERK biosensor activity
Basedon
Experiment
LocalID
Figure3F_timelapse_BRET
XYZ-scale
XY: 0.867 micrometer/pixel
T-scale
2 minites for each time interval
dblink
None
External links
-
Source
None
BDML
None
PDPML
None
Experiment type
TimeLapse
Microscope type
FluorescenceMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
IX81, Olympus
Detector model
None
Objective model
UPLAPO 100X/1.35NA oil
Filter set
None
Sample
-
Dye
-
Clearing
-
Biological Process
activation of MAPERK kinase
(
GO:0000186
)
Cellular Component
plasma membrane
(
GO:0005886
)
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