Cell membrane images, restored images, and segmentation results of 52 C. elegans embryos.
Description
We performed 3D timelapse imaging of 52 embryos, in which the cell membrane is labeled with green fluorescent protein (GFP), for two hours from the two-cell stage. An image restoration method based on deep learning was applied to improve the image quality. An image processing method called BCOMS (Biologically Constrained Optimization-based cell Membrane Segmentation) was applied and the cellular regions were segmented.