Whole-mount fluorescence in situ hybridization of Osiris family gene (http://flybase.org/reports/FBgg0000612) probes on the pupal head (42 hour after puparium formation). Class 1 genes (Osiris3, 7, 9, 22, mainly expressed in epidermal cells), Class 2 genes (Osiris 1, 3, 4, 5, 7, 6, 8, 9, 11, 12, 13, 16, 21, 22, 23, 24, sensory organs). The data set is 5 channel, 3 dimentional images of whole-mount fluorescence in situ hybridization. X-Y: 1024x 768 pixel (1.1710 µm/pixel), Z: 15µm/slice. For Osi 4, 5, 6, 8, 11, 12, 13, 16, 21, 23, 24, channel 1: DAPI; channel 2 anti-phosphorylated tyrosin antibody, Alexa 488; channel 3: Osiris gene probe, Cy3; channel 4 anti-Futsch (22C10) antibody Alexa 647; channel 5 transmitted light. For Osi 1, 7, 9, channel 2: anti-Futsch (22C10) antibody Alexa 488; channel 4, chitin binding probe (cbp) Alexa647. Files starting with "STD_" are 2D projection of each stack.
See details in "Sun Z, Inagaki S, Miyoshi K, Saito K, Hayashi S. Osiris gene family defines the cuticle nanopatterns of Drosophila. Genetics. 2024 Jun 5;227(2):iyae065. doi: 10.1093/genetics/iyae065. PMID: 38652268; PMCID: PMC11151929."