Description
Whole-mount fluorescence in situ hybridization of Osiris family gene (http://flybase.org/reports/FBgg0000612) probes on the pupal head (42 hour after puparium formation). Class 1 genes (Osiris3, 7, 9, 22, mainly expressed in epidermal cells), Class 2 genes (Osiris 1, 3, 4, 5, 7, 6, 8, 9, 11, 12, 13, 16, 21, 22, 23, 24, sensory organs). The data set is 5 channel, 3 dimentional images of whole-mount fluorescence in situ hybridization. X-Y: 1024x 768 pixel (1.1710 µm/pixel), Z: 15µm/slice. For Osi 4, 5, 6, 8, 11, 12, 13, 16, 21, 23, 24, channel 1: DAPI; channel 2 anti-phosphorylated tyrosin antibody, Alexa 488; channel 3: Osiris gene probe, Cy3; channel 4 anti-Futsch (22C10) antibody Alexa 647; channel 5 transmitted light. For Osi 1, 7, 9, channel 2: anti-Futsch (22C10) antibody Alexa 488; channel 4, chitin binding probe (cbp) Alexa647. Files starting with "STD_" are 2D projection of each stacke.