SSBD:database

GSE24775_PP_LD-Delay_rep2

Project
9-Masumoto-Transcriptome
Dataset
GSE24775_PP_LD-Delay_rep2
Title
OmicsBDML file of Genome-wide expression analysis of the mouse pars tuberalis (PT) under chronic long-day condition (delay condition)
Version
0.18
OmicsBDML ID
7eaa0664-7fb7-440f-97b4-1be25263acf7
Release
2014-10-03
License
CC BY-SA
Description
Gene expression data from mice pars tuberalis in the long day condition (delay condition)
Datatype
gene expression data
Organism
Mus musculus
Local ID
GSE24775_PP_LD-Delay_rep2
Based On
Experiment
Contributors text
Hiroki R. Ueda, Koh-hei Masumoto, Maki Ukai-Tadenuma, Takeya Kasukawa, Mamoru Nagano, Kenichiro D. Uno, Kaori Tsujino, Kazumasa Horikawa, Yasufumi Shigeyoshi
PMID
21129973
dblink
http://brainstarts.org
Methods summary
See details in Masumoto et al. (2010) Curr Biol 20, 2199-2206.
Data Kind
data
tScale
4.0
tUnit
hour
Contact
Hiroki R. Ueda
RIKEN
Center for Developmental Biology
Functional Genomics Unit
Affiliation
RIKEN
Center for Developmental Biology
Functional Genomics Unit
Contributors
Hiroki R Ueda
RIKEN
Koh-hei Masumoto
Maki Ukai-Tadenuma
Takeya Kasukawa
Mamoru Nagano
Kenichiro D Uno
Kaori Tsujino
Kazumasa Horikawa
Yasufumi Shigeyoshi
MINiML Series
Accession: GSE24775
Title: Genome-wide expression analysis of the mouse pars tuberalis (PT) under chronic short-day and long-day conditions
Type: Expression profiling by array
Pubmed-ID: 21129973
Living organisms detect seasonal changes in day length (photoperiod), and alter their physiological functions accordingly, to fit seasonal environmental changes. This photoperiodic system is implicated in seasonal affective disorders and the season-associated symptoms observed in bipolar disease and schizophrenia. Thyroid-stimulating hormone beta subunit (Tshb), induced in the pars tuberalis (PT), plays a key role in the pathway that regulates animal photoperiodism. However, the upstream inducers of Tshb expression remain unknown. Here we show that late-night light stimulation acutely triggers the Eya3-Six1 pathway, which directly induces Tshb expression. Using melatonin-proficient CBA/N mice, which preserve the photoperiodic Tshb-expression response, we performed a genome-wide expression analysis of the PT under chronic short-day and long-day conditions. These data comprehensively identified long-day and short-day genes, and indicated that late-night light stimulation induces long-day genes. We verified this by advancing and extending the light period by 8 hours, which acutely induced Tshb expression, within one day. In a genome-wide expression analysis under this condition, we searched for candidate upstream genes by looking for expression that preceded Tshb’s, and identified Eya3 gene. These results elucidate the comprehensive transcriptional photoperiodic response in the PT, revealing the complex regulation of Tshb expression and unexpectedly rapid response to light changes in the mammalian photoperiodic system.
Mice were separated into 2 groups. One group was maintained under the short-day conditions (light: dark = 8 h:16 h, ZT0 = lights on, ZT8 = lights off, 400 lux) and the other was housed under long-day conditions (light:dark = 16 h:8 h, ZT0 = lights on, ZT16 = lights off, 400 lux) for 2 weeks. The PTs of both groups were retrieved every 4 h for 1 day (6 time points for each group), starting at ZT0. For the experiments performed during the first day of the long-day conditions, we applied two different conditions, following 3 weeks under short-day conditions. In one, the light-onset was advanced by 8 hours (advance condition), and in the other, the dark period was delayed by 8 hours (delay condition). PTs from both groups were obtained every 4 h for 1 day, starting at the lights-on time. (Lights on for the advance condition was ZT16 as defined by the short-day condition. Lights on for the delay condition was ZT0). We sampled 25 mice at each time point. This whole procedure was repeated twice (n = 2) to obtain experimental replicates.
Sample refs: GSM610250, GSM610251, GSM610252, GSM610253, GSM610254, GSM610255, GSM610256, GSM610257, GSM610258, GSM610259, GSM610260, GSM610261, GSM610262, GSM610263, GSM610264, GSM610265, GSM610266, GSM610267, GSM610268, GSM610269, GSM610270, GSM610271, GSM610272, GSM610273, GSM610274, GSM610275, GSM610276, GSM610277, GSM610278, GSM610279, GSM610280, GSM610281, GSM610282, GSM610283, GSM610284, GSM610285, GSM610286, GSM610287, GSM610288, GSM610290, GSM610292, GSM610294, GSM610296, GSM610297, GSM610298, GSM610299, GSM610300, GSM610301
MINiML Database
Gene Expression Omnibus (GEO)
NCBI NLM NIH
http://www.ncbi.nlm.nih.gov/geo
Platform(s)
GPL1261
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
in situ oligonucleotide
Mus musculus
Affymetrix
Data payload
{'sample_count': 6, 'object_count': 1, 'component_count': 6, 'sample_refs': ['GSM610250', 'GSM610251', 'GSM610252', 'GSM610253', 'GSM610254', 'GSM610255', 'GSM610256', 'GSM610257', 'GSM610258', 'GSM610259', 'GSM610260', 'GSM610261', 'GSM610262', 'GSM610263', 'GSM610264', 'GSM610265', 'GSM610266', 'GSM610267', 'GSM610268', 'GSM610269', 'GSM610270', 'GSM610271', 'GSM610272', 'GSM610273', 'GSM610274', 'GSM610275', 'GSM610276', 'GSM610277', 'GSM610278', 'GSM610279', 'GSM610280', 'GSM610281', 'GSM610282', 'GSM610283', 'GSM610284', 'GSM610285', 'GSM610286', 'GSM610287', 'GSM610288', 'GSM610290', 'GSM610292', 'GSM610294', 'GSM610296', 'GSM610297', 'GSM610298', 'GSM610299', 'GSM610300', 'GSM610301'], 'samples': [{'iid': 'GSM610287', 'title': 'PP LD-Delay 700, rep2', 'accession': 'GSM610287', 'accession_database': 'GEO', 'organism': 'Mus musculus', 'organism_taxid': '10090', 'source': 'long day delay, ZT0', 'molecule': 'total RNA', 'description': 'Gene expression data from mice pars tuberalis at ZT0 in the long day delay condition.\nPooled samples from 25 mice.', 'platform_ref': 'GPL1261', 'contact_ref': 'contrib1', 'external_data_rows': '45101', 'external_data_uri': 'GSM610287-tbl-1.txt', 'characteristics': [{'tag': 'strain', 'value': 'CBA/N'}, {'tag': 'gender', 'value': 'male'}, {'tag': 'tissue', 'value': 'pars tuberalis'}, {'tag': 'age', 'value': '6 weeks'}, {'tag': 'condition', 'value': 'long day delay'}, {'tag': 'time point', 'value': 'ZT0'}]}, {'iid': 'GSM610290', 'title': 'PP LD-Delay 1100, rep2', 'accession': 'GSM610290', 'accession_database': 'GEO', 'organism': 'Mus musculus', 'organism_taxid': '10090', 'source': 'long day delay, ZT4', 'molecule': 'total RNA', 'description': 'Gene expression data from mice pars tuberalis at ZT4 in the long day delay condition.\nPooled samples from 25 mice.', 'platform_ref': 'GPL1261', 'contact_ref': 'contrib1', 'external_data_rows': '45101', 'external_data_uri': 'GSM610290-tbl-1.txt', 'characteristics': [{'tag': 'strain', 'value': 'CBA/N'}, {'tag': 'gender', 'value': 'male'}, {'tag': 'tissue', 'value': 'pars tuberalis'}, {'tag': 'age', 'value': '6 weeks'}, {'tag': 'condition', 'value': 'long day delay'}, {'tag': 'time point', 'value': 'ZT4'}]}, {'iid': 'GSM610294', 'title': 'PP LD-Delay 1500, rep2', 'accession': 'GSM610294', 'accession_database': 'GEO', 'organism': 'Mus musculus', 'organism_taxid': '10090', 'source': 'long day delay, ZT8', 'molecule': 'total RNA', 'description': 'Gene expression data from mice pars tuberalis at ZT8 in the long day delay condition.\nPooled samples from 25 mice.', 'platform_ref': 'GPL1261', 'contact_ref': 'contrib1', 'external_data_rows': '45101', 'external_data_uri': 'GSM610294-tbl-1.txt', 'characteristics': [{'tag': 'strain', 'value': 'CBA/N'}, {'tag': 'gender', 'value': 'male'}, {'tag': 'tissue', 'value': 'pars tuberalis'}, {'tag': 'age', 'value': '6 weeks'}, {'tag': 'condition', 'value': 'long day delay'}, {'tag': 'time point', 'value': 'ZT8'}]}, {'iid': 'GSM610297', 'title': 'PP LD-Delay 1900, rep2', 'accession': 'GSM610297', 'accession_database': 'GEO', 'organism': 'Mus musculus', 'organism_taxid': '10090', 'source': 'long day delay, ZT12', 'molecule': 'total RNA', 'description': 'Gene expression data from mice pars tuberalis at ZT12 in the long day delay condition.\nPooled samples from 25 mice.', 'platform_ref': 'GPL1261', 'contact_ref': 'contrib1', 'external_data_rows': '45101', 'external_data_uri': 'GSM610297-tbl-1.txt', 'characteristics': [{'tag': 'strain', 'value': 'CBA/N'}, {'tag': 'gender', 'value': 'male'}, {'tag': 'tissue', 'value': 'pars tuberalis'}, {'tag': 'age', 'value': '6 weeks'}, {'tag': 'condition', 'value': 'long day delay'}, {'tag': 'time point', 'value': 'ZT12'}]}, {'iid': 'GSM610299', 'title': 'PP LD-Delay 2300, rep2', 'accession': 'GSM610299', 'accession_database': 'GEO', 'organism': 'Mus musculus', 'organism_taxid': '10090', 'source': 'long day delay, ZT16', 'molecule': 'total RNA', 'description': 'Gene expression data from mice pars tuberalis at ZT16 in the long day delay condition.\nPooled samples from 25 mice.', 'platform_ref': 'GPL1261', 'contact_ref': 'contrib1', 'external_data_rows': '45101', 'external_data_uri': 'GSM610299-tbl-1.txt', 'characteristics': [{'tag': 'strain', 'value': 'CBA/N'}, {'tag': 'gender', 'value': 'male'}, {'tag': 'tissue', 'value': 'pars tuberalis'}, {'tag': 'age', 'value': '6 weeks'}, {'tag': 'condition', 'value': 'long day delay'}, {'tag': 'time point', 'value': 'ZT16'}]}, {'iid': 'GSM610301', 'title': 'PP LD-Delay 300, rep2', 'accession': 'GSM610301', 'accession_database': 'GEO', 'organism': 'Mus musculus', 'organism_taxid': '10090', 'source': 'long day delay, ZT20', 'molecule': 'total RNA', 'description': 'Gene expression data from mice pars tuberalis at ZT20 in the long day delay condition.\nPooled samples from 25 mice.', 'platform_ref': 'GPL1261', 'contact_ref': 'contrib1', 'external_data_rows': '45101', 'external_data_uri': 'GSM610301-tbl-1.txt', 'characteristics': [{'tag': 'strain', 'value': 'CBA/N'}, {'tag': 'gender', 'value': 'male'}, {'tag': 'tissue', 'value': 'pars tuberalis'}, {'tag': 'age', 'value': '6 weeks'}, {'tag': 'condition', 'value': 'long day delay'}, {'tag': 'time point', 'value': 'ZT20'}]}]}