XY: 0.554 micrometer/pixel, Z: 2.187 micrometer/slice, XY: 0.083026 micrometer/pixel, Z: NA micrometer/slice, XY: 1.2525 micrometer/pixel, Z: 1 micrometer/slice, XY: 0.065273 micrometer/pixel, Z: NA micrometer/slice, XY: 0.55 micrometer/pixel, Z: NA micrometer/slice
T scale
30 minutes for each time interval, 0.327 second for each time interval, 0.315 second for each time interval, 0.417 second for each time interval, 4.91 second for each time interval, 30 minutes for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Carl Zeiss LSM780
Detector model
-
Objective model
-
Filter set
-
Related paper(s)
Ritsuko Morita, Miho Kihira, Yousuke Nakatsu, Yohei Nomoto, Miho Ogawa, Kazumasa Ohashi, Kensaku Mizuno, Tetsuhiko Tachikawa, Yukitaka Ishimoto, Yoshihiro Morishita, Takashi Tsuji (2016) Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations., PloS one, Volume 11, Number 9, pp. e0161336
Published in 2016
(Electronic publication in Sept. 2, 2016, midnight )
(Abstract) The morphologies of ectodermal organs are shaped by appropriate combinations of several deformation modes, such as invagination and anisotropic tissue elongation. However, how multicellular dynamics are coordinated during deformation processes remains to be elucidated. Here, we developed a four-dimensional (4D) analysis system for tracking cell movement and division at a single-cell resolution in developing tooth epithelium. The expression patterns of a Fucci probe clarified the region- and stage-specific cell cycle patterns within the tooth germ, which were in good agreement with the pattern of the volume growth rate estimated from tissue-level deformation analysis. Cellular motility was higher in the regions with higher growth rates, while the mitotic orientation was significantly biased along the direction of tissue elongation in the epithelium. Further, these spatio-temporal patterns of cellular dynamics and tissue-level deformation were highly correlated with that of the activity of cofilin, which is an actin depolymerization factor, suggesting that the coordination of cellular dynamics via actin remodeling plays an important role in tooth epithelial morphogenesis. Our system enhances the understanding of how cellular behaviors are coordinated during ectodermal organogenesis, which cannot be observed from histological analyses.