SSBD:database

Summary of 77-Aoki-CellDynERK

SSBD:database
77-Aoki-CellDynERK
SSBD:database URL
https://ssbd.riken.jp/database/77/
Title
Time-lapse imgaes and quantitative information of propagating wave of ERK activation orients collective cell migration
Description
-
Relase date
2018-11-14
Updated date
2022-11-21
Errata
2022/11/21 Corrected the organism to Canis lupus familiaris/MDCK cell from Mus musculus by author's request.
License
CC BY
Kind
Quantitative data, Image data based on Experiment
Number of Datasets
16 ( Image datasets: 14, Quantitative data datasets: 2 )
Size of Datasets
1.4 GB ( Image datasets: 1.4 GB, Quantitative data datasets: 548.1 KB )
Organism(s)
Canis lupus familiaris
Cell lines(s)
MDCK cell
Protein name(s)
CRaf, ERK, H2B, KRasCT, MCL, MEK
Protein tag(s)
mCherry
Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
activation of MAP ERK kinase kinase
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
XY: 0.91 micrometer/pixel, Z: NA, XY: 0.65 micrometer/pixel, Z: NA, XY: 0.65 micrometer/pixel, Z: NA , XY: 0.49 micrometer/pixel, Z: NA, XY: 0.49 micrometer/pixel, Z: NA , XY: 1.48 micrometer/pixel, Z: NA, XY: 1.48 micrometer/pixel, Z: NA
T scale
180 second for each time interval, 120 second for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
FluorescenceMicroscope
Acquisition mode
BrightField
Contrast method
Fluorescence
Microscope model
Olympus IX81
Detector model
Photometrics,Tucson,AZ
Objective model
UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.43, NA, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.38, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.35, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.45, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.48, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.39, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.40, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.44, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.46, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.36, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.41, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.37, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.47, UPlanSApo 20x/0.75 or UJPlanSApo 60x/1.42
Filter set
FF01-438/24(Semrock, Rochester, NY);FF01-542/27(Semrock), NA, FF01-641/75(Semrock);FF01-692/LP(Semrock), FF01-580/20(Semrock);FF01-632/22(Semrock), FF01-438/24(Semrock, Rochester, NY);FF01-483/32(Semrock)
Related paper(s)

Kazuhiro Aoki, Yohei Kondo, Honda Naoki, Toru Hiratsuka, Reina E Itoh, Michiyuki Matsuda (2017) Propagating Wave of ERK Activation Orients Collective Cell Migration., Developmental cell, Volume 43, Number 3, pp. 305-317.e5

Published in 2017 Nov 6

(Abstract) The biophysical framework of collective cell migration has been extensively investigated in recent years; however, it remains elusive how chemical inputs from neighboring cells are integrated to coordinate the collective movement. Here, we provide evidence that propagation waves of extracellular signal-related kinase (ERK) mitogen-activated protein kinase activation determine the direction of the collective cell migration. A wound-healing assay of Mardin-Darby canine kidney (MDCK) epithelial cells revealed two distinct types of ERK activation wave, a "tidal wave" from the wound, and a self-organized "spontaneous wave" in regions distant from the wound. In both cases, MDCK cells collectively migrated against the direction of the ERK activation wave. The inhibition of ERK activation propagation suppressed collective cell migration. An ERK activation wave spatiotemporally controlled actomyosin contraction and cell density. Furthermore, an optogenetic ERK activation wave reproduced the collective cell migration. These data provide new mechanistic insight into how cells sense the direction of collective cell migration.
(MeSH Terms)
Contact
Kazuhiro Aoki , National Institutes of Natural Sciences , National Institute for Basic Biology , Division of Quantitative Biology
Contributors
Kazuhiro Aoki, Yohei Kondo, Honda Naoki, Toru Hiratsuka, Reina E. Itoh, Michiyuki Matsuda

Dataset List of 77-Aoki-CellDynERK

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 4019
Datast ID Figure1B
Dataset Kind Image data
Dataset Size 784.9 MB
4D view
SSBD:OMERO
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# 4020
Datast ID Figure1G
Dataset Kind Image data
Dataset Size 49.3 MB
4D view
SSBD:OMERO
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# 4021
Datast ID Figure4A
Dataset Kind Image data
Dataset Size 241.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 4022
Datast ID Figure4A_ERK
Dataset Kind Image data
Dataset Size 3.6 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 4023
Datast ID Figure4A_merge
Dataset Kind Image data
Dataset Size 3.6 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 4024
Datast ID Figure4A_MLC
Dataset Kind Image data
Dataset Size 3.6 MB
4D view
SSBD:OMERO
Download BDML
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# 4025
Datast ID Figure5A
Dataset Kind Image data
Dataset Size 1.7 MB
4D view
SSBD:OMERO
Download BDML
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# 4026
Datast ID Figure6B_iRFP
Dataset Kind Image data
Dataset Size 24.8 MB
4D view
SSBD:OMERO
Download BDML
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# 4027
Datast ID Figure6B_mCherry_ERK
Dataset Kind Image data
Dataset Size 24.8 MB
4D view
SSBD:OMERO
Download BDML
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# 4028
Datast ID Figure6C_opticflow
Dataset Kind Image data
Dataset Size 7.4 MB
4D view
SSBD:OMERO
Download BDML
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# 4029
Datast ID FigureS1A_ADAM10_inhibitor
Dataset Kind Image data
Dataset Size 128.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 4030
Datast ID FigureS1A_DMSO
Dataset Kind Image data
Dataset Size 128.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 4031
Datast ID FigureS4_Beads
Dataset Kind Image data
Dataset Size 1.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 4032
Datast ID FigureS4_Ratio
Dataset Kind Image data
Dataset Size 1.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data
# 4033
Datast ID Figure4C
Dataset Kind Quantitative data
Dataset Size 548.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data