SSBD:database

Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai (2013) SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction., Nature neuroscience, Volume 16, Number 8, pp. 1154-61

Published in 2013 Aug (Electronic publication in June 23, 2013, midnight )

• doi: 10.1038/nn.3447
• pmid: 23792946
  

(Abstract) We report a water-based optical clearing agent, SeeDB, which clears fixed brain samples in a few days without quenching many types of fluorescent dyes, including fluorescent proteins and lipophilic neuronal tracers. Our method maintained a constant sample volume during the clearing procedure, an important factor for keeping cellular morphology intact, and facilitated the quantitative reconstruction of neuronal circuits. Combined with two-photon microscopy and an optimized objective lens, we were able to image the mouse brain from the dorsal to the ventral side. We used SeeDB to describe the near-complete wiring diagram of sister mitral cells associated with a common glomerulus in the mouse olfactory bulb. We found the diversity of dendrite wiring patterns among sister mitral cells, and our results provide an anatomical basis for non-redundant odor coding by these neurons. Our simple and efficient method is useful for imaging intact morphological architecture at large scales in both the adult and developing brains.

• Animals
• Benzoates/pharmacology
• Benzyl Alcohol/pharmacology
• Brain/drug effects
• Brain/embryology
• Brain/ultrastructure[major]
• Dendrites/ultrastructure
• Electroporation
• Female
• Fixatives/pharmacology
• Fluorescent Dyes/analysis[major]
• Fluorescent Dyes/chemistry
• Formaldehyde/pharmacology
• Fructose/chemistry
• Fructose/pharmacology[major]
• Gene Knock-In Techniques
• Glycerol/analogs & derivatives[major]
• Glycerol/chemistry
• Glycerol/pharmacology
• Histocytological Preparation Techniques[major]
• Light
• Mice
• Mice, Inbred C57BL
• Mice, Inbred ICR
• Mice, Transgenic
• Microscopy/methods
• Nerve Net/ultrastructure[major]
• Neurons/ultrastructure
• Odorants
• Olfactory Bulb/cytology
• Olfactory Bulb/drug effects
• Olfactory Perception/physiology
• Phenyl Ethers/pharmacology
• Polymers/pharmacology
• Pregnancy
• Scattering, Radiation
• Solutions/chemistry
• Solutions/pharmacology[major]
• Sucrose/pharmacology
• Tissue Fixation/methods
• Urea/pharmacology