Summary of 188-Ipponjima-NHEKsCornification

SSBD:database
SSBD:database URL
Title
Images of morphological changes in granular cells during cornification.
Description
-
Relase date
2022-03-31
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
21 ( Image datasets: 21, Quantitative data datasets: 0 )
Size of Datasets
265.3 GB ( Image datasets: 265.3 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Homo sapiens
Reporter(s)
CTB, CTG, CYTO-ID, Hoechst 33258, LTR, MBG, MTR, TMRE

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
mitochondrial depolarization, keratinocyte activation
Cellular Component (CC)
nucleus, NA
Biological Imaging Method
two-photon laser scanning microscopy, time lapse microscopy
X scale
0.223 micrometer/pixel, 0.208 micrometer/pixel, 0.1563 micrometer/pixel
Y scale
0.223 micrometer/pixel, 0.208 micrometer/pixel, 0.1563 micrometer/pixel
Z scale
0.5 micrometer/slice, 0.6 micrometer/slice
T scale
NA, 90 second for each time interval, 120 second for each time interval, 4 hour for time interval, 180 second for each time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Sari Ipponjima, Yuki Umino, Masaharu Nagayama, Mitsuhiro Denda (2020) Live imaging of alterations in cellular morphology and organelles during cornification using an epidermal equivalent model., Scientific reports, Volume 10, Number 1, pp. 5515

Published in 2020 Mar 26 (Electronic publication in March 26, 2020, midnight )

(Abstract) The stratum corneum plays a crucial role in epidermal barrier function. Various changes occur in granular cells at the uppermost stratum granulosum during cornification. To understand the temporal details of this process, we visualized the cell shape and organelles of cornifying keratinocytes in a living human epidermal equivalent model. Three-dimensional time-lapse imaging with a two-photon microscope revealed that the granular cells did not simply flatten but first temporarily expanded in thickness just before flattening during cornification. Moreover, before expansion, intracellular vesicles abruptly stopped moving, and mitochondria were depolarized. When mitochondrial morphology and quantity were assessed, granular cells with fewer, mostly punctate mitochondria tended to transition to corneocytes. Several minutes after flattening, DNA leakage from the nucleus was visualized. We also observed extension of the cell-flattening time induced by the suppression of filaggrin expression. Overall, we successfully visualized the time-course of cornification, which describes temporal relationships between alterations in the transition from granular cells to corneocytes.
(MeSH Terms)

Contact
Sari Ipponjima , Hokkaido University , Research Center of Mathematics for Social Creativity, Research Institute for Electronic Science
Contributors


Dataset List of 188-Ipponjima-NHEKsCornification

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 7381
Dataset Kind Image data
Dataset Size 424.7 MB
4D view
SSBD:OMERO
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# 7382
Dataset Kind Image data
Dataset Size 25.5 GB
4D view
SSBD:OMERO
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# 7383
Dataset Kind Image data
Dataset Size 30.2 GB
4D view
SSBD:OMERO
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# 7384
Dataset Kind Image data
Dataset Size 528.6 MB
4D view
SSBD:OMERO
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# 7385
Dataset Kind Image data
Dataset Size 49.1 GB
4D view
SSBD:OMERO
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# 7386
Dataset Kind Image data
Dataset Size 6.2 GB
4D view
SSBD:OMERO
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# 7387
Dataset Kind Image data
Dataset Size 5.9 GB
4D view
SSBD:OMERO
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# 7388
Dataset Kind Image data
Dataset Size 34.1 GB
4D view
SSBD:OMERO
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# 7389
Dataset Kind Image data
Dataset Size 38.7 GB
4D view
SSBD:OMERO
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# 7390
Dataset Kind Image data
Dataset Size 17.4 GB
4D view
SSBD:OMERO
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# 7391
Dataset Kind Image data
Dataset Size 28.7 GB
4D view
SSBD:OMERO
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# 7392
Dataset Kind Image data
Dataset Size 28.2 GB
4D view
SSBD:OMERO
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# 7393
Datast ID FigureS7_MBG_5min
Dataset Kind Image data
Dataset Size 50.5 MB
4D view
SSBD:OMERO
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# 7394
Datast ID FigureS7_MBG_30min
Dataset Kind Image data
Dataset Size 27.5 MB
4D view
SSBD:OMERO
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# 7395
Dataset Kind Image data
Dataset Size 21.4 MB
4D view
SSBD:OMERO
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# 7396
Datast ID FigureS7_MTR_5min
Dataset Kind Image data
Dataset Size 16.4 MB
4D view
SSBD:OMERO
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# 7397
Datast ID FigureS7_MTR_30min
Dataset Kind Image data
Dataset Size 16.9 MB
4D view
SSBD:OMERO
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# 7398
Dataset Kind Image data
Dataset Size 17.9 MB
4D view
SSBD:OMERO
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# 7399
Datast ID FigureS7_TMRE_5min
Dataset Kind Image data
Dataset Size 46.9 MB
4D view
SSBD:OMERO
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# 7400
Dataset Kind Image data
Dataset Size 43.9 MB
4D view
SSBD:OMERO
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# 7401
Dataset Kind Image data
Dataset Size 30.4 MB
4D view
SSBD:OMERO
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