SSBD:database

Key Organism Strain Cell Line Gene Symbol Protein name Protein tag Reporter Project ID Dataset ID Title Description Data Type Gene Ontology Biological Imaging Methods Method Summary Paper Information Medical Subject Headlines Person

Value

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178-konagaya-CellMigration

https://ssbd.riken.jp/database/178-konagaya-CellMigration/

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ssbd-repos-000178

https://ssbd.riken.jp/repository/178/

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Images and BDML file for quantitative information of ERK activity in myogenic cell migration during mouse muscle regneration.

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2022-03-31

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CC BY

Image data based on Experiment

160 ( Image datasets: 160, Quantitative data datasets: 0 )

55.5 GB ( Image datasets: 55.5 GB, Quantitative data datasets: 0 bytes )

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Mus musculus

CDK1 inhibitor, CDK1_2 inhibitor, CDK4_6 inhibitor, MEK inhibitor

EKAREV, EKAREV-NLS, Fucci, mVenus-hGeminin, tdKeima

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extracellular signal-regulated kinase activity

cell migration

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two-photon laser scanning microscopy, differential interference contrast microscopy, time lapse microscopy, fluorescence microscopy

0.397 micrometer/pixel, 1.325 micrometer/pixel, 1.59 micrometer/pixel, 0.621 micrometer/pixel, 0.795 micrometer/pixel, 1.2034 micrometer/pixel, 1.988 micrometer/pixel

0.397 micrometer/pixel, 1.325 micrometer/pixel, 1.59 micrometer/pixel, 0.621 micrometer/pixel, 0.795 micrometer/pixel, 1.2034 micrometer/pixel, 1.988 micrometer/pixel

1 micrometer/slice, 2 micrometer/slice, 24 micrometer/slice, 10 micrometer/slice, 5 micrometer/slice

267 sec for each time interval, 283 sec for each time interval, 314 sec for each time interval, 306 sec per time interval, 290 sec per time interval, 268.32 sec per time interval, 341 sec for each time interval, 300 sec per time interval, 259 sec for each time interval, 272 sec for each time interval, 274 sec per time interval, 285 sec for each time interval, 1 sec per time interval, NA, 238.06 sec per time interval, 305 sec per time interval, 287 sec for each time interval, 314.85 sec per time interval, 295 sec for each time interval, 300 sec for each time interval, 316 sec for each time interval, 266 sec per time interval, 278.2 sec per time interval, 308 sec per time interval, 284 sec per time interval, 270.06 sec per time interval, 311 sec per time interval, 306 sec for each time interval, 92 sec for each time interval, 320 sec for each time interval, 322 sec per time interval, 319 sec per time interval, 308 sec for each time interval, 264 sec per time interval, 328 sec per time interval, 258 sec for each time interval, 265 sec for each time interval, 382 sec for each time interval, 307 sec per time interval, 294 sec per time interval, 273 sec for each time interval, 319 sec for each time interval, 277 sec for each time interval, 278 sec per time interval, 234 sec per time interval, 1 sec for each time interval, 314 sec per time interval, 313 sec per time interval, 307 sec for each time interval, 282 sec for each time interval, 291 sec for each time interval, 240 sec per time interval, 297 sec for each time interval, 237 sec for each time interval, 312 sec per time interval, 260 sec for each time interval, 310 sec for each time interval, 292 sec for each time interval, 328.2 sec per time interval, 284 sec for each time interval, 303 sec for each time interval, 309 sec for each time interval, 263 sec for each time interval, 288 sec per time interval, 286 sec for each time interval, 301 sec for each time interval, 284.5 sec per time interval, 281 sec per time interval, 258 sec per time interval, 280 sec for each time interval, 267 sec per time interval

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Yumi Konagaya, Kanako Takakura, Maina Sogabe, Anjali Bisaria, Chad Liu, Tobias Meyer, Atsuko Sehara-Fujisawa, Michiyuki Matsuda, Kenta Terai (2020) Intravital imaging reveals cell cycle-dependent myogenic cell migration during muscle regeneration., Cell cycle (Georgetown, Tex.), Volume 19, Number 22, pp. 3167-3181

Published in 2020 Nov (Electronic publication in Nov. 1, 2020, midnight )

• doi: 10.1080/15384101.2020.1838779
• pmid: 33131406
  

(Abstract) During muscle regeneration, extracellular signal-regulated kinase (ERK) promotes both proliferation and migration. However, the relationship between proliferation and migration is poorly understood in this context. To elucidate this complex relationship on a physiological level, we established an intravital imaging system for measuring ERK activity, migration speed, and cell-cycle phases in mouse muscle satellite cell-derived myogenic cells. We found that in vivo, ERK is maximally activated in myogenic cells two days after injury, and this is then followed by increases in cell number and motility. With limited effects of ERK activity on migration on an acute timescale, we hypothesized that ERK increases migration speed in the later phase by promoting cell-cycle progression. Our cell-cycle analysis further revealed that in myogenic cells, ERK activity is critical for G1/S transition, and cells migrate more rapidly in S/G2 phase 3 days after injury. Finally, migration speed of myogenic cells was suppressed after CDK1/2-but not CDK1-inhibitor treatment, demonstrating a critical role of CDK2 in myogenic cell migration. Overall, our study demonstrates that in myogenic cells, the ERK-CDK2 axis promotes not only G1/S transition but also migration, thus providing a novel mechanism for efficient muscle regeneration.

(MeSH Terms)

• Animals
• Cardiotoxins/adverse effects
• Cell Cycle/genetics[major]
• Cell Line
• Cell Movement/genetics[major]
• Cell Proliferation/genetics
• Cyclin-Dependent Kinase 2/metabolism
• Epithelial Cells/metabolism
• Extracellular Signal-Regulated MAP Kinases/metabolism
• Female
• Humans
• Intravital Microscopy/methods[major]
• MAP Kinase Signaling System/genetics
• Male
• Mice
• Mice, Transgenic
• Muscle Development/genetics
• Muscle Fibers, Skeletal/metabolism
• Muscle, Skeletal/diagnostic imaging[major]
• Muscle, Skeletal/drug effects
• Muscle, Skeletal/injuries
• Muscle, Skeletal/physiology[major]
• Regeneration/genetics[major]
• Satellite Cells, Skeletal Muscle/metabolism[major]
• Transfection

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Kenta Terai , Graduate School of Medicine, Kyoto University , Department of Pathology and Biology of Diseases , Department of Pathology and Biology of Diseases

Yumi Konagaya

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